Vector Labs
Fluorescein Avidin DCS
A-2011
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In this experiment, we wanted to detect the co-localization of H2AX and telomere-associated foci. Therefore, Fluorescein Avidin DCS was used to increase the fluorescent signaling of our primary antibody. This conjugate is reliable and improved staining specificity.
Immunofluorescence
Mouse
Primary antibody of interest (produced in rabbit)
0.1% BSA + Normal Goat serum (1:60)
Goat anti-rabbit IgG (H+L) Biotinylated (Vector Lab)
Avidin DCS-fluorescein (1:500; 20 min)
Fluorescence
Specific staining, with higher sensitivity and lower background
High reproducibility
None