Bright CD20 Antibody for B cells

February 04, 2022

BlueskyReddit
Pathology
UMass
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Beckman Coulter

Product Name:

CD20-PC7, B9E9, 1 mL, ASR

Catalog Number:

IM3629U

Clone Number:

B9E9

Info:Supplier Page View Product Specs

Browse Similar Products: CD20 Antibodies

CD20, a marker used for the identification of mature B cells, can be downregulated and can appear dim, especially in cases of Chronic Lymphocytic Leukemia (CLL). Therefore, labs will often need a CD20 conjugated to bright fluorochrome. This antibody is just that, but it comes with a few problems.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human lymphocytes

Primary Incubation

15 min @ RT

Blocking Agent

1% FBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Blue laser (in FL-5, the PE-Cy7 channel)

Results Summary

Although the antibody stained the B cells well (shown in blue), the negative population appeared spread out a lot. This makes it harder to resolve dim populations when CD20 is downregulated. In addition, the positive cells are very bright, which can cause terrible compensation issues in nearby channels, e.g. PE, ECD and PC5.

DOI or PMID #

Additional Notes

Related Categories

Image Gallery

Summary

The Good

Strong signal, bright positive cells.

The Bad

Wide negative peak, compensation issues

The Bottom Line

This is a good antibody when used on its own, or when used in combination with antibodies that are not labelled with fluorochromes in nearby channels, which renders compensation less of an issue.

Share your experience with other scientists. Write a Review! »

Join the discussion