Issues with Detecting SRp40 in Keratinocytes

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Company:

Santa Cruz Biotechnology, Inc

Product Name:

Anti-SR Antibody (1H4)

Catalog Number:

sc-13509

Clone Number:

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Aim was to detect nuclear SRp40 in keratinocytes. The antibody did not work in immunofluorescence staining application.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Oral keratinocytes

Primary Incubation

1/25-100, 4C overnight

Blocking Agent

5% goat serum

Secondary Incubation

1/500, 1h, goat anti-mouse IgG H&L

Tertiary Incubation

DAPI for nuclear counter staining

Detection

Alexa Fluor 488

Results Summary

We did not get any positive staining in the staining. We may need to try it Western blot.

DOI or PMID #

Additional Notes

Related Categories

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Summary

The Good

None

The Bad

It did not work in the staining.

The Bottom Line

Need to try in Western blot. It may work.

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