SR (1H4) Antibody sc-13509 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemSR (1H4) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-13509
Antibody TypePrimary Antibodies
NCBI Gene AliasesSF2, SRp30b, SRp20, SRp30a, SC-35, SRP55, B52, SFRS6, HEL-S-91, SFRS4, SFRS3, SFRS2, SFRS1, ASF, SRP75, SFRS2A, HRS, SF2p33, SRP40, SC35, PR264, SFRS5
Quantity200 µg/ml
ApplicationsWB, IP, IF, IHC(P), ELISA
Clone1H4
Reactivityhuman
Conjugate/TagUnconjugated
ClonalityMonoclonal
Hostmouse
FormatPurified
NCBI Full Gene Nameserine and arginine rich splicing factor 2, serine and arginine rich splicing factor 3, serine and arginine rich splicing factor 1, serine and arginine rich splicing factor 6, serine and arginine rich splicing factor 4, serine and arginine rich splicing factor 5
TargetSR
IsotypeIgG1

Questions and Answers

Can this antibody be used to study the role of SRSF1 in cancer, particularly in relation to angiogenesis?

Yes, the SR (1H4) Antibody has been utilized in studies examining the role of SRSF1 in cancer. For instance, in research involving WT1 mutants, SRSF1 was shown to regulate the splicing of VEGF, which is crucial for angiogenesis. The inhibition of SRSF1 or its associated kinase SRPK1 was found to reverse altered VEGF splicing, thereby impacting tumor growth and angiogenesis ().

Is this antibody effective for immunoprecipitation studies?

The SR (1H4) Antibody has been successfully employed in immunoprecipitation studies to analyze phosphorylated SRSF1. In a study, cell lysates were incubated with this antibody to detect phosphorylated forms of SRSF1, demonstrating its utility in such applications ().

What applications has this antibody been shown to work in?

The SR (1H4) Antibody has been documented for use in various applications including Western Blotting, ELISA, Immunofluorescence, Immunohistochemistry, and Immunoprecipitation. Its versatility makes it suitable for a range of experimental setups in cell biology ().

Does this antibody interact with other splicing factors in the context of alternative splicing?

Yes, the SR (1H4) Antibody has been referenced in studies that explore interactions with other splicing factors. For example, it was used alongside other antibodies to investigate the role of splicing factors in regulating alternative splice site selection, indicating its relevance in studies of splicing mechanisms ().

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) Nuclear speckles enable processing of RNA from GC-rich isochoresCellApril 2, 2026Michał Małszycki, Lisa Martina, İbrahim Avşar Ilık, Daniela Salgado Salgado Figueroa, Nirmalya Dasgupta, Menşura Feray Çoşar, Keun-Tae Kim, Gil Carraco, Beatrix Fauler, David Meierhofer, et al.incubated with Dynabeads Protein G beads pre-bound to p-SR antibody (10μL per IP, 1H4, Santa Cruz, sc-13509) for 90 minutes in the cold-room. The supernatant from 1H4 immunoprecipitation is used for DHX9 immunoprecipitation

(2) Influenza A virus NS1 effector domain is required for PA-X-mediated host shutoff in infected cellsJournal of virologyMay 14, 2024Bougon J, Kadijk E, Gallot-Lavallee L, Curtis BA, Landers M, Archibald JM, Khaperskyy DA.ab75855), SON (1:600, rabbit, Abcam, ab121759), and SR proteins (1:100, mouse, Santa Cruz Biotechnology, sc-13509). Alexa Fluor (AF)-conjugated secondary antibodies used were as follows: donkey anti-mouse IgG AF488

(3) Modulation of RNA splicing enhances response to BCL2 inhibition in leukemiaCancer cellJanuary 9, 2023Wang E, Pineda JMB, Kim WJ, Chen S, Bourcier J, Stahl M, Hogg SJ, Bewersdorf JP, Han C, Singer ME, et al.

(4) SPHINX-Based Combination Therapy as a Potential Novel Treatment Strategy for Acute Myeloid LeukaemiaBritish journal of biomedical scienceJanuary 1, 2023Wodi C, Belali T, Morse R, Porazinski S, Ladomery M.primary antibodies: anti-SRPK1 (EE-13, Santa Cruz Biotechnology; 1:1000) or anti-pan-SR-1H4 antibody (sc-13509, Santa Cruz Biotechnology; 1:500), anti-β-actin (ab8226, Abcam UK; 1:5000). Membranes were incubated

(5) The natural drug DIAVIT is protective in a type II mouse model of diabetic nephropathyPloS oneJanuary 1, 2019Stevens M, Neal CR, Craciun EC, Dronca M, Harper SJ, Oltean S.

(6) A role for SOX9 in post-transcriptional processes: insights from the amphibian oocyteScientific reportsMay 8, 2018Penrad-Mobayed M, Perrin C, L'Hôte D, Contremoulins V, Lepesant JA, Boizet-Bonhoure B, Poulat F, Baudin X, Veitia RA.

(7) Hypoxia leads to significant changes in alternative splicing and elevated expression of CLK splice factor kinases in PC3 prostate cancer cellsBMC cancerApril 2, 2018Bowler E, Porazinski S, Uzor S, Thibault P, Durand M, Lapointe E, Rouschop KMA, Hancock J, Wilson I, Ladomery M.

(8) Body Temperature Cycles Control Rhythmic Alternative Splicing in MammalsMolecular cellAugust 3, 2017Preußner M, Goldammer G, Neumann A, Haltenhof T, Rautenstrauch P, Müller-McNicoll M, Heyd F.

(9) Myelodysplasia-associated mutations in serine/arginine-rich splicing factor SRSF2 lead to alternative splicing of CDC25CBMC molecular biologyAugust 23, 2016Skrdlant L, Stark JM, Lin RJ.

(10) Enhanced SRSF5 Protein Expression Reinforces Lamin A mRNA Production in HeLa Cells and Fibroblasts of Progeria PatientsHuman mutationMarch 1, 2016Vautrot V, Aigueperse C, Oillo-Blanloeil F, Hupont S, Stevenin J, Branlant C, Behm-Ansmant I.

(11) Modulation of alternative splicing by expression of small nuclear ribonucleoprotein polypeptide NThe FEBS journalDecember 1, 2014Lee MS, Lin YS, Deng YF, Hsu WT, Shen CC, Cheng YH, Huang YT, Li C.

(12) HnRNP L and hnRNP LL antagonistically modulate PTB-mediated splicing suppression of CHRNA1 pre-mRNAScientific reportsOctober 14, 2013Rahman MA, Masuda A, Ohe K, Ito M, Hutchinson DO, Mayeda A, Engel AG, Ohno K.anti-His-tag (D293-1, Medical & Biological Laboratories), anti-SF2/ASF (Zymed), anti-SR proteins 1H4 (sc-13509, Santa Cruz Biotechnology), anti-GAPDH (Sigma-Aldrich), anti-β-actin C4 (sc-47778, Santa Cruz Biotechnology),

(13) WT1 mutants reveal SRPK1 to be a downstream angiogenesis target by altering VEGF splicingCancer cellDecember 13, 2011Amin EM, Oltean S, Hua J, Gammons MV, Hamdollah-Zadeh M, Welsh GI, Cheung MK, Ni L, Kase S, Rennel ES, et al.were incubated with mouse anti-SRSF1 (Santa Cruz SC-33562) or anti-Panphospho-SR antibody (Santa Cruz, SC-13509) and Protein G Dynabeads (Invitrogen). To detect phosphorylated SRSF1, the eluent was immunoblotted

(14) Serine/arginine-rich protein 30c activates human papillomavirus type 16 L1 mRNA expression via a bimodal mechanismThe Journal of general virologyOctober 1, 2011Somberg M, Li X, Johansson C, Orru B, Chang R, Rush M, Fay J, Ryan F, Schwartz S.

(15) Increased expression of cellular RNA-binding proteins in HPV-induced neoplasia and cervical cancerJournal of medical virologyMay 1, 2009Fay J, Kelehan P, Lambkin H, Schwartz S.

(16) Increased dosage of Dyrk1A alters alternative splicing factor (ASF)-regulated alternative splicing of tau in Down syndromeThe Journal of biological chemistryOctober 17, 2008Shi J, Zhang T, Zhou C, Chohan MO, Gu X, Wegiel J, Zhou J, Hwang YW, Iqbal K, Grundke-Iqbal I, et al.

(17) Lark is the splicing factor RBM4 and exhibits unique subnuclear localization propertiesDNA and cell biologyAugust 1, 2006Markus MA, Morris BJ.

(18) XE7: a novel splicing factor that interacts with ASF/SF2 and ZNF265Nucleic acids researchJanuary 1, 2006Mangs AH, Speirs HJ, Goy C, Adams DJ, Markus MA, Morris BJ.peptide antigen on Sepharose-4B beads followed by column chromatography. Mouse monoclonal anti-SR (1H4; sc-13509) was from Santa Cruz and rabbit polyclonal anti-haemagglutinin (HA) was from Clontech. Mouse monoclonal

(19) HADHB, HuR, and CP1 bind to the distal 3'-untranslated region of human renin mRNA and differentially modulate renin expressionThe Journal of biological chemistryNovember 7, 2003Adams DJ, Beveridge DJ, van der Weyden L, Mangs H, Leedman PJ, Morris BJ.

(20) Association of herpes simplex virus type 1 ICP8 and ICP27 proteins with cellular RNA polymerase II holoenzymeJournal of virologyJune 1, 2002Zhou C, Knipe DM.

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(1)

1/12/2022Issues with Detecting SRp40 in Keratinocytes Lin ChenAim was to detect nuclear SRp40 in keratinocytes. The antibody did not work in immunofluorescence staining application.

SR (1H4) Antibody

SR (1H4) Antibody from Santa Cruz Biotechnology, Inc.

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Questions and Answers

Santa Cruz Biotechnology, Inc.

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SR (1H4) Antibody from Santa Cruz Biotechnology, Inc.

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