Biolegend
anti-CD192 PerCP/Cy5.5
357203
K036C2
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This antibody was used to stain for CCR2 expression on monocytes in human peripheral blood. It was chosen because it is a commonly used clone for CCR2, PerCP/Cy5.5 works well with other fluorophores in the staining panel, and Biolegend has a good reputation for making quality flow cytometry antibodies.
Flow Cytometry
whole blood
5 ul per test in 100uL staining volume for 15 minutes at room temperature
FcBlock + 0.2% BSA
N/A
LSR II
CD192 staining was excellent (filled histogram) as compared to unstained monocytes (dotted line).
Works well. Reproducible results.
Will continue to use this antibody.