Fig 2: Phagocytosis of SNECs in human blood neutrophils and monocytes is negatively correlated with the oxidative burst. (A, B) Peripheral blood from individuals with systemic lupus erythematosus (SLE; n = 13), chronic granulomatous disease (CGD; n = 3) and from normal healthy donors (NHD; n = 8) was incubated with human PBMC-derived SNECs for 3 h. Phagocytosis indices (PhIx) of CD14-CD16+CCR2- neutrophils (A) and CD14+CD16-/LOCCR2+ monocytes (B) were determined by flow cytometry. Patients with SLE exhibited significant accumulation of SNECs. Graphs show individual values, means ± S.E.M. **p < 0.01, ***p < 0.001, as determined by Student's t-test. (C, D) Correlation analysis between spontaneous intracellular production of ROS (DHR-123 MFI) and phagocytosis indices in blood CD14-CD16+CCR2- neutrophils (C) and CD14+CD16-/LOCCR2+ monocytes (D). (E, F) Correlation analysis between levels of anti-dsDNA autoantibodies and PhIx in blood CD14-CD16+CCR2- neutrophils (E) and CD14+CD16-/LOCCR2+ monocytes (F). Pearson coefficients (r) and p values (determined by 2-tailed Student's t-test) in C-F are depicted within the graphs.