Solid CD16 Antibody

January 05, 2022

BlueskyReddit
Pathology
UMass
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Beckman Coulter

Product Name:

CD16-APC-A750, 3G8, 0.5 mL, ASR

Catalog Number:

A66330

Clone Number:

3G8

Info: Supplier Page View Product Specs

Browse Similar Products: CD16 Antibodies

CD16 is expressed on granulocytes and on NK cells (lymphocytes). Although the identification of grans is relative easy using a FSC/SSC plot, there are occasions when a good granulocytic marker is needed (e.g. in MDS). Also, NK cell identification is better when both CD56 and CD16 are included. We got this antibody from Beckman to help in both the above tasks.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Blood / PBMC

Primary Incubation

2uL per test (only!)

Blocking Agent

1% foetal bovine serum

Secondary Incubation

None

Tertiary Incubation

None

Detection

Navios EX flow cytometer

Results Summary

The results show excellent separation of grans and NK cells from other blood cells. This was achieved even with only 2uL per test! The antibody binds well, and has a bright signal (as shown in the histogram). One needs to be careful though about spillage into neighbouring channels, and tandem breakdown. Keep an eye on the signal in the APC channel.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Strong signal. Only 2uL per test is enough.

The Bad

Expensive! Spills into APC.

The Bottom Line

This is a good, reliable antibody. It is ASR, so that adds to the quality and reliability. But it comes at a price. Coulter charge too much for their ASR antibodies.

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