abcam
DNMT3L
ab93613
N117/9
Info:Supplier Page View Product Specs
Browse Similar Products: CD3 Flow Cytometry Antibodies
This antibody is acceptable for use in flow cytometry after biotin conjugation but does not perform exceptionally well.
Flow Cytometry
Mouse splenocytes
Fc block @ 1:100, 30 minutes RT; Fix/perm 20 minutes 4C
2.4G2
Add biotinylated antibody 1:100, 30 minutes RT
Add SA-APC 1:1600, 30 minutes RT
Flow cytometry (Attune)
After biotinylation, DNMT3L expressing cells are detectable by flow cytometry with SA-APC, although there is a continuous range of intermediate to highly stained cells.
Unlabeled antibody was conjugated to biotin using abeam's lightning link type A kit before staining
One of the few commercially available DNMT3L antibodies
Not extremely clean staining
Reasonable for use flow cytometry although somewhat dirty and expensive for its results