Reliable Conjugation Kit

University of Toronto
Chemical/Biomedical Engineering
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

abcam

Product Name:

Biotin Conjugation Kit (Fast, Type A)

Catalog Number:

ab201795

While a little expensive, this kit is extremely easy to use and reliably biotinylates antibodies for use in flow cytometry. It takes less than half an hour of incubation time, and less than 1 minute of preparation time to conjugate any unlabeled antibody in an easy single step conjugation without any further optimization required.

Experimental Design and Results Summary

Application

Custom labeling of antibodies for flow cytometry

Starting Material

Tips

No tips necessary, mixing the reagent with your antibody exactly as the manufacturer specifies is sufficient for optimal performance.

Results Summary

Reliable labeling of unlabeled antibodies for use with fluorophore conjugated SA in downstream flow cytometry experiments. No loss of antibody function was noticed due to over labeling.

DOI or PMID #

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Summary

The Good

Minimal time and effort required for good performance

The Bad

Expensive

The Bottom Line

Well worth the price for efficient and quick biotinylation of antibodies.

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