Detects Hsp70 but Some Bleedover

R&D
EydisBio Inc.
Principal Investigator

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies

Product Name:

HSP70 (D69) Antibody

Catalog Number:

4876

Clone Number:

N/A

Info: Supplier Page View Product Specs

Browse Similar Products: Hsp70 Antibodies

This antibody detected Hsp70 from primate blood samples but there is some bleeding from one sample to the next which presents as un even bands.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Primate serum

Primary Incubation

1:2000

Blocking Agent

NDS

Secondary Incubation

1:2000

Tertiary Incubation

N/A

Detection

Chemilumescence

Results Summary

This antibody detected Hsp70 expression but some optimization is needed to get clean bands via western blot. Although there is one band detected it has some bleed over and may need to be optimized for future use or publication.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Detects Hsp70

The Bad

Need optimization

The Bottom Line

This is a good antibody for Hsp70 detection.

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