Mouse Foxp3 EF450 Gives Good Results

Surgery
UCSF
Graduate Student
November 29, 2021

BlueskyReddit
Surgery
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher

Product Name:

FOXP3 Monoclonal Antibody (FJK-16s), eFluor 450

Catalog Number:

48-5773-82

Clone Number:

N/A

Info: Supplier Page View Product Specs

Browse Similar Products: Anti-FOXP3 Antibodies

We have used this antibody to identify regulatory T cells in the thymus upon perturbation.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse thymus

Primary Incubation

1:200 dilution for 30 minutes at room temp

Blocking Agent

2.4G2 Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSRII FACS Analyzer

Results Summary

We used this antibody to show that about 25% of CD73+ cells in the thymus are also Foxp3+ Tregs.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Stains cleanly enough for an intracellular stain

The Bad

Rather expensive and not great separation.

The Bottom Line

Is a decent choice for staining Foxp3, we will use again

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