Merck
Anti-Tyrosine Hydroxylase Antibody
AB152
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I have used this antibody to detect dopaminergic neurons in the brain. It has worked very well in sample of mouse.
Immunofluorescence
Mouse brain
Incubation was done with Ab diluted to 1:1,000 for 16h at 4ºC
BSA 4% in PBS
Incubation was done with 2nd Ab diluted to 1:1,000 for 1h at room temperature
None
Fluorescence (AF 647)
In all cases reactivity is quite good at 1:1,000 dilution. Within the VTA (ventral segmentar area), this protein is detected only in dopaminergic neurons and thus can be used to differentiate these neurons. I have captured this image in my confocal to show expression of this protein in the mouse VTA (please see my paper for details).
30782975
Reliable and sensible
Great antibody