Great yH2AX for Western Blot

October 28, 2021

Department of Internal Medicine
East Tennessee State University
Graduate Student

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Company:

Cell Signaling

Product Name:

Phospho-Histone H2A.X (Ser139) () Rabbit

Catalog Number:

9718S

Clone Number:

20E3

The DNA damage during HIV infection was accessed using yH2AX expression levels. HIV infection increased DNA damage.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human T cells

Primary Incubation

1:1000

Blocking Agent

5% Milk

Secondary Incubation

1:1000

Tertiary Incubation

N/A

Detection

Western Blot (Chemiluminescence)

Results Summary

HIV infection increased levels of yH2AX. DNA damage is increased during HIV infections.

DOI or PMID #

https://doi.org/10.1128/JVI.01061-20

Additional Notes

N/A

Image Gallery

Summary

The Good

Good antibody.

The Bad

Difficult to detect. Use more protein quantity.

The Bottom Line

Significant differences in levels of yH2AX were seen during HIV infection. Will use it again.

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