Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb 9718S from Cell Signaling Technology

Product Specs
ItemPhospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb
CompanyCell Signaling Technology
Price $410.00 Inquire
Catalog Number9718S
Quantity100 µl
ApplicationsW, IHC-Bond, IHC-P, IF-IC, FC-FP
ReactivityH, M, R, Mk
TargetH2AX (Ser139) phosphate
ModificationsH2AX (Ser139) phosphate
HostRabbit
NCBI Gene AliasesH2A.X, H2A/X, H2AFX
ClonalityMonoclonal
NCBI Full Gene NameH2A.X variant histone
Research AreaChromatin Regulation / Nuclear Function
IsotypeIgG
Clone20000
Molecular Weight15

Questions and Answers

Can this antibody be used to detect DNA damage in cancer cells?

Yes, the Phospho-Histone H2A.X (Ser139) Rabbit mAb has been utilized in various studies to detect DNA double-strand breaks, which are indicative of DNA damage in cancer cells. For instance, it has been employed in immunofluorescence assays to visualize and quantify DNA damage events in cultured human cancer cells ().

What is the recommended dilution for using this antibody in immunofluorescence?

The recommended dilution for the Phospho-Histone H2A.X (Ser139) antibody in immunofluorescence applications is 1:1000, as noted in multiple studies that have successfully employed this antibody for detecting phosphorylated H2A.X in various experimental setups (, ).

Is this antibody effective for use in flow cytometry?

Yes, the Phospho-Histone H2A.X (Ser139) antibody has been shown to be effective for use in flow cytometry. It has been utilized in studies to analyze drug-induced DNA damage responses in various cell types, confirming its applicability in this technique ().

What species can this antibody react with?

The Phospho-Histone H2A.X (Ser139) antibody is reactive with multiple species, including human, mouse, rat, and non-human primates, making it versatile for use in various biological research contexts ().

Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) TFEB confers resistance against the chemotherapeutic agent CX-5461Autophagy ReportsDecember 31, 2026Marjorie Rolland, Benoît Marchand, Laure Bessy, Florence McDuff, Marie-Josée Boucher390,849), GAPDH (Cell Signaling Technology, 2118), p-H2AXSer139 (H2AX) (Cell Signaling Technology, 9718), HISTONE H3 (Cell Signaling Technology, 3638), LC3B (Cell Signaling Technology, 3868), TFEB (Cell Signaling Technology

(2) Design, synthesis and anti-breast cancer activity evaluation of 6,7-dihydro-5 H -pyrrolo[3,4- d ]pyrimidine-based PARP1/ATR dual inhibitorsJournal of Enzyme Inhibition and Medicinal ChemistryDecember 31, 2026Meng-Lan He, Zong-Hao Wang, Xia Yao, Lu-Lu Ye, Bo-Qun Du, Chen-Chen Wang, Yong-Hao Chen, Xiao-Xian Wang, Hui Luo, Yuan Gao, et al.overnight at 4 °C with rabbit anti-γH2AX monoclonal antibody (1:1000; Cell Signalling Technology, #9718). After three PBS washes, Alexa Fluor 488-conjugated goat anti-rabbit IgG (H + L) (1:3000; Invitrogen,

(3) Targeted inhibition of ATR kinase promotes antitumor immunity in HNSCC through enhanced STING activation and T cell functionTranslational OncologySeptember 1, 2026Fabio HB da Costa, Mutsuki Kawabe, Tsung-You Tsai, Kento Okamoto, Mason D. Bartels, Sofia Cortes, Ratna Veeramachaneni, Roberto Rangel, Mitchell J. Frederick, Vlad C. Sandulache, et al.from mice (1:100, #sc-58,749, Santa Cruz Biotechnology), Histone H2A.X (Ser139) (20E3, 1:200, cat# 9718, Cell Signaling); overnight at 4 °C followed by Alexa Fluor 594-conjugated secondary antibodies, respectively.

(4) ACSS2 is required for colorectal cancer progression and a druggable target for colorectal cancer treatmentiScienceAugust 21, 2026Lei Wang, Urszula Dougherty, Wenliang He, Lu Gao, Caleb Muefong, Rajesh Sarkar, Jie Du, Ardaman Shergill, Hening Lin, Marc Bissonnette, et al.AB_10597084" Anti-H3K27ac,Milipore,"Cat#: MABE647, RRID: AB_2893037" Anti-p-Histone H2AX,Cell Signaling Technology,"Cat#: 9718S, RRID:AB_2118009" Anti-p-ACSS2-Ser659,Signalway antibody,Cat#: 58003 Anti-beta-catenin,ProteinTech,"Cat#:

(5) Progressive suppression of DNA repair genes with persistent p53 target activation in doxorubicin-treated cardiomyocytesG3: Genes, Genomes, GeneticsAugust 5, 2026Emma M Pfortmiller, José A Gutiérrez, Alyssa R Bogar, Michelle C Warddirected against β-Actin 1:1000 (4970S, Cell Signaling Technology, Danvers, MA, USA), γH2AX 1:1000 (9718S, Cell Signaling Technology), and p53 1:200 (sc-126, Santa Cruz Biotechnology, Dallas, TX, USA) diluted

(6) SDF-1 Attenuates Oocyte Quality Decline During Reproductive Aging Through Autophagy-Enhanced Stress Granule ScavengingAdv Sci (Weinh)August 3, 2026Rui Long, Meng Wang, Ruolin Mao, Hong Chen, Juepu Zhou, Xiangfei Wang, Zheng Yuan, Ri-Cheng Chian, Nan Xiao, Qingsong Xi, et al.antibody, 1:100, Cell Signaling, 86298; anti‐rabbit phospho‐histone H2AX antibody, 1:200, Cell Signaling, 9718; anti‐rabbit LC3A/B antibody, 1:200, Cell Signaling, 12741; anti‐rabbit Beclin‐1 antibody, 1:200, Abcam,

(7) Targeting RPRD1B overcomes chemoresistance in gastric cancer by suppressing the TOPBP1-mediated DNA damage repair pathwayCell Oncol (Dordr)August 2, 2026Jian Sheng, Jianghua Li, Chenxi Cao, Xiaorong Liu, Chunhua He, Mingjian Fei, Bin Wu, Xiangli Li, Chundong Hu, Shumin Liu, et al.cat. no. PA5-148238), anti-TOPBP1 (1:1,000; CST, cat. no. 14342), anti-p-γH2AX (1:1,000; CST, cat. no. 9718), and β-Actin (1:3,000; Proteintech, cat. no. 23660-1-AP). The corresponding secondary antibody was

(8) Activation of the Lactate Receptor GPR81 Ameliorates Senescence Hallmarks and Improves Muscle Function in Cellular and Progeroid Models of AgingAging CellAugust 2, 2026Pihu Mehrotra, Sai Harsha Bhamidipati, Pedro Lei, John Toftegaard, Debanik Choudhury, Maryam Elsayed, Yali Zhang, Jianmin Wang, Shweta Chitkara, G. Ekin Atilla-Gokcumen, et al.western blot),Company GPR81,NLS2095 PA5‐67873,ICC: 1:200 WB: 1:1000,Novus Biotech Invitrogen ϒH2AX (Phospho‐Histone H2A.X (Ser139)),9718,ICC: 1:100,Cell Signaling Technology P21,10355‐1‐AP,ICC: 1:200,Proteintech Ki67,ab245113,ICC: 1:200,Abcam MYH1,05‐716,ICC:

(9) Cell cycle checkpoint activity in the malaria parasite Plasmodium falciparummSphereJuly 28, 2026Monique K. Johnson, Juliana Naldoni, William H. Lewis, Ross F. Waller, Catherine J. Merrick1:200, with secondary goat anti-rabbit HRP 1:5,000 (Abcam, ab97080); or rabbit α-γ-H2aX (Cell signaling, 9718S) with goat α-rabbit IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 594 (Invitrogen, A-11012).

(10) HELZ-BRCA2 complex resolves R-loops to drive transcription-coupled homologous recombinationNat CommunJuly 23, 2026Wenjing Li, Bo Wu, Boya Gao, Elizabeth M. Irvin, Arijit Ghosh, Lillian Eliaz, Yuxin Huang, Youngho Kwon, Clara M. Stiefel, Tram Thi Ngoc Nguyen, et al.(T1989) (30632, Cell Signaling; 1:1000), p-Chk1 (S345) (2348, Cell Signaling; 1:1000), and γH2AX (S139) (9718, Cell Signaling; 1:1000), according to the instructions provided by the manufacturers. If needed, the

(11) The Marine Triterpene Stellettin B Triggers Mitochondrial-to-Nuclear Translocation of AIF/EndoG and Reverses Epithelial-Mesenchymal Transition to Inhibit Oral Cancer ProgressionInt J Med SciJuly 22, 2026Peng-Yu Chen, Pin-Chen Kuo, Yi-Lung Ling, Yen-Yun Wang, Shyng-Shiou F. Yuan, Jyh-Horng Sheu, Wan-Chi TsaiSanta Cruz), Endonuclease G (1:1000, 4969S, Cell Signaling Technology), phospho-Histone H2A.X (1:1000, 9718S, Cell Signaling Technology), Slug (1:1000, 9585S, CST), Snail (1:2000, 3895S, Cell Signaling Technology),

(12) Long non-coding RNA LOC101926915-encoded micropeptide carboplatin-induced upregulation factor confers carboplatin resistance in ovarian cancerCytojournalJuly 21, 2026Man Li, YingRu Xu, ZhiBiao Mai, Liangliang Ren, Xuexin Yu, SuWen Chen, ZunFu Ke, Ruyuan Yufor 1 h, cells were incubated at 4°C with the primary antibodies targeting gamma-H2AX (γ-H2AX) (Cat#9718, Cell Signaling Technology, Danvers, MA, USA) at 1:2000 dilution or HATag at 1:300 dilution (Cat#3724,

(13) Sulfonylated Goniothalamin and Piplartine Derivatives Exhibit Selective Antiproliferative Activity on Breast Cancer Cells via Oxidative Stress-Mediated MechanismsACS OmegaJuly 21, 2026Julia Louise Moreira Nacif, Aloisio de Andrade Bartolomeu, Simone da Silva Lamartine-Hanemann, Bruno Zavan, Luiza de Mello Nascimento, Ester Siqueira Caixeta, Alexandre Ferro Aissa, Ronaldo Aloise Pilli, Marisa Iontaprimary antibodies: anti-α-tubulin (1:100, Cell Signaling Technology, #3873) or anti-γH2AX (1:100, CST, #9718). The next day, cells were incubated with secondary antibodies for 2 h at room temperature: antimouse

(14) Targeted antibody-drug conjugates for rhabdomyosarcoma and other FGFR4-expressing cancersCell Rep MedJuly 21, 2026Meijie Tian, Katrina Jia, Jerry T. Wu, Adam T. Cheuk, Siteng Fang, Victor T. Ojo, Eleanor G. Pope, David Milewski, Yong Yean Kim, Hsien-Chao Chou, et al.133D3, Cat# 2348, RRID: AB_331212), and anti-phospho-Histone H2A.X (Ser139) (1:500, clone 20E3, Cat# 9718S, RRID: AB_2118009), all from Cell Signaling Technology. Loading control antibodies included anti-β-actin

(15) Pharmacological chromatin remodeling enhances response to estrogen therapy in ER + breast cancerMol OncolJuly 17, 2026Anneka L. Johnson Thomas, Barbara Karakyriakou, Patricia C. Muskus, Alyssa M. Roberts, Justin Rendleman, Alexa Warren, Hanxu Lu, Kimberly A. Birmingham, Malone K. Friedman, Abigail E. Goen, et al.for 1 h at room temp. Primary antibody (phospho‐histone H2AXS139, γH2AX, Cell Signaling Technology #9718) was diluted 1 : 300 in blocking buffer and incubated with cells on a slow rocker at 4 °C overnight.

(16) Erk inhibitors intercept Erk-mediated negative feedback while imposing cell-cycle arrest and activating p38iScienceJuly 17, 2026Merav Darash-Yahana, Nadine Soudah, Alexey Baskin, Inbal Levy-Saar, Alexander Levitzki, David EngelbergRRID: AB_628073 Rabbit monoclonal anti phosphor-histone H2A.X (Ser139),Cell Signaling Technology,Cat#9718; RRID: AB_2118009 Rabbit monoclonal anti cleaved caspase-3 (Asp175),Cell Signaling Technology,Cat#9664;

(17) Genome instability is increased during DNA replication in the presence of the CPD UV photoproduct in transcriptionally active genesiScienceJuly 17, 2026Rika Kusumoto-Matsuo, Yusuke Matsuno, Naoki Akiba, Yuya Manaka, Jiro Toshima, Fumio Hanaoka, Ken-Ichi Yoshiokainhibitors Antibodies against the following proteins and nucleotides were obtained from the indicated suppliers: γH2AX (9718, Cell Signaling Technology, Danvers, MA, USA; 613402, BioLegend, San Diego, CA, USA), 53BP1 (PC712,

(18) Histone H3.3 ensures cell proliferation and genomic stability during myeloid cell developmentiScienceJuly 17, 2026Sakshi Chauhan, Fuki Kudoh, Anup Dey, Keiko OzatoRRID:AB_2535731 Rabbit monoclonal anti-phospho-Histone H2A.X (Ser139),Cell Signaling Technology,Cat # 9718; RRID:AB_2118009 "Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™

(19) The WEE1 inhibitor azenosertib broadly enhances efficacy of antibody-drug conjugates with topoisomerase I and microtubule inhibitor payloadsiScienceJuly 17, 2026Xiao Guo, Doris Kim, Olivier Harismendy, Erika Cabrera, Heekyung Chung, Funda Meric-Bernstam, Mark R. Lackner, Catherine Lee, Jianhui MaAB_2713924 Rabbit monoclonal anti-Phospho-Histone H2A.X (Ser139) (20E3),Cell Signaling Technology,Cat# 9718S; RRID: AB_211800 Rabbit monoclonal anti-Phospho-Histone H3 (Ser10) (D7N8E),Cell Signaling Technology,Cat#

(20) VLX1570 suppresses neuroblastoma growth through inhibition of PCNA-associated proliferative signaling and potentiates cisplatin antitumor activityFront PharmacolJuly 15, 2026Wei-Hsun Yang, Chong-Sun Khoi, Kuan-Lin Kuo, Po-Ming Chow, Chen-Hsun Hsu, Yi-Ju Kao, Wei-Chou Lin, Shang-Jen Chang, Shih-Ming Liao, Felicia Tai, et al.(#9541), Bcl-2 (#15071), PCNA (#13110), phospho-histone H3 (#53348), and phospho-histone H2A.X (Ser139) (#9718) (all from Cell Signaling Technology, Danvers, MA, USA); β-actin (#sc-69879), p21 (#sc-397), and p53

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(1)

10/24/2023Average Phospho-Histone H2A.X From Cell Signaling I RIbeiroPhospho-Histone H2A.X (H2A histone proteins) antibody was used in rat peripheral blood mononuclear cells (PBMCs) samples.
(1)

10/28/2021Great yH2AX for Western Blot Sushant KhanalThe DNA damage during HIV infection was accessed using yH2AX expression levels. HIV infection increased DNA damage.

Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb

Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb from Cell Signaling Technology

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