Beckman Coulter
CD3-PB, UCHT1, 0.5 mL, ASR
A93687
UCHT1
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CD3 is the archetypic marker for identification of T cells. We needed a good CD3 antibody on the violet laser to stain T cells with high quality.
Flow Cytometry
Human WBC prep
10uL for 15 min at Room Temp
None
N/A
Navios 10-Color Flow Cytometer
The results were acceptable, showing decent separation of CD3+ vs. CD3- cells; however, the staining was dim. As shown in the picture, the Median Fluorescence Intensity (MFI) is quite low for the positive cells. This is OK in most cases, but ideally the resolution should be better. Brighter violet dyes are available, and so my advice is to use a different antibody.
Easy to optimize
Dim signal
A decent antibody, but not recommended when a strong signal is needed. Other violet dyes will produce stronger signals. Check other suppliers before you buy.