Desmin Rb mAb (for IHC) from Abclonal

October 01, 2021

BlueskyReddit
Rheumatology and Clinical Immunology
University Medical Center Groningen
PhD candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

ABclonal

Product Name:

Desmin Rabbit mAb

Catalog Number:

A3736

Clone Number:

N/A

Desmin is a specific marker for vascular smooth muscle cells. The antibody reviewed here gave specific and best results in immunohistochemical staining in human artery biopsy.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Human artery

Primary Incubation

1:200, 1 hour at room temperature (Antigen retrieval at pH9 for 15min first)

Blocking Agent

1% BSA PBS

Secondary Incubation

DAKO P448 1:50, 40 min

Tertiary Incubation

No

Detection

DAKO DAB 1:50, 4 min

Results Summary

The antibody works very well in IHC staining. The staining is very specific, positive only in vascular smooth muscle cells (media in blood vessel), negative in the adventitia and the intima. And only minimal background.

DOI or PMID #

No

Additional Notes

No

Related Categories

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Summary

The Good

Specific for vascular smooth muscle cells, little background

The Bad

Overstained easily, should optimize the staining protocol first

The Bottom Line

The antibody works very well in IHC staining. The staining is very specific and the background is very clean. The antibody is at good price with high quality.

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