Good IP Magnetic Beads

September 15, 2021

BlueskyReddit
Stanley Manne Children’s Research Institute
Molecular and Translational Cancer Biology
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Pierce™ Protein A/G Magnetic Beads

Catalog Number:

88803

The lab focus is on the study of pediatric leukemia in the context of JAK-STAT signaling. We are interested in developing new therapies and treatments for this disease. We are also interested in drug resistant leukemia.

Experimental Design and Results Summary

Application

Immunoprecipitation

Starting Material

Cell lysate

Tips

TBST washes the beads well

Results Summary

This product allowed for a great IP of the target protein. I got great pulldown.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Easy to use with both protein A/G

The Bad

Tricky to optimize your IP protocol

The Bottom Line

This is a good magnetic bead that has both protein A/G so you can use multiple antibody types with your immunoprecipitation. I found that a wash buffer with tween does a better job of washing the beads. It is still a bit tricky to optimize your IP though. This product does have decently low background binding which is nice.

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