Perfect for Cell Purification

University of Toronto
Chemical/Biomedical Engineering
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

LS coluns

Catalog Number:

130-042-401

Info: Supplier Page 

We study the mechanisms responsible for establishing and maintaining immune cell tolerance

Experimental Design and Results Summary

Application

Negative selection of cells labeled with magnetic beads (typically CD3 depletion)

Starting Material

Mouse/human thymus and lymph nodes

Tips

Load sample by splitting it into many smaller volumes rather than one large volume all at once

Results Summary

Fantastic separation of the magnetically labeled "dump" population of cells to be discarded from the unlabeled cells to be used for downstream analysis

DOI or PMID #

N/A

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Summary

The Good

Works very well for negative selection

The Bad

Very expensive

The Bottom Line

A pricey product but worth the cost for clean depletion of cells labeled with magnetic beads

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