Cheap Viability Dye

University of Toronto
Chemical/Biomedical Engineering
PhD Candidate

Overall

Quality of Results

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Company:

Biolegend

Product Name:

Zombie NIR fixable viability kit

Catalog Number:

423105

We study the mechanisms responsible for establishing and maintaining immune cell tolerance in the thymus and the periphery

Experimental Design and Results Summary

Application

Viability stain for flow cytometry and FACS

Starting Material

Primary cells from mouse/human thymus and lymph nodes

Tips

Extra wash steps can sometimes help lower background

Results Summary

Good staining in most cases with extra wash steps, but live/dead separation can sometimes be messy

DOI or PMID #

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Summary

The Good

Cheap and still good for most applications

The Bad

Can be a dirtier stain when precision is needed

The Bottom Line

Good for general purpose flow when other gates will help clean up samples

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