Lambda Light Chain for B Cells

September 20, 2021

BlueskyReddit
Pathology
UMass
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

PE Mouse Anti-Human Lambda

Catalog Number:

642919

Clone Number:

1-155-2

Identifying lambda-restricted B cells is critical in the diagnosis of B cell malignancies. The selection of anti-light chain specific antibodies needs to be done carefully. We chose this antibody because it is recommended by other scientists.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Blood

Primary Incubation

20 minutes at room temp, followed by 2 washes

Blocking Agent

Serum proteins

Secondary Incubation

None

Tertiary Incubation

None

Detection

575/30 filter

Results Summary

Results show good staining on B cells that express the lambda light chain of immunoglobulins. The rest of the lymphocytes appear (as they should be) negative. Very little nonspecific staining is seen.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Strong staining. Specific. Monoclonal.

The Bad

Need 20uL per test.

The Bottom Line

This is a good antibody. It shows specific staining, but is needed at 20uL per test. This is a bit too much, and other antibodies may produce the same results using lesser volumes.

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