Kappa Antibody Works Well for Clonality Determination

September 14, 2021

BlueskyReddit
Pathology
UMass
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

FITC Mouse Anti-Human Kappa

Catalog Number:

643773

Clone Number:

TB28-2

Info: Supplier Page View Product Specs

Browse Similar Products: Anti-Kappa Antibodies

In the study of B cells (and plasma cells), it is important to identify if they are clonal. The best evidence for that is the expression of immunoglobulins that express either kappa or lambda light chains. This is why having an anti-kappa antibody is important.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Blood or bone marrow

Primary Incubation

No dilution. Use 20uL per test. Incubate for 20 minutes

Blocking Agent

Fetal calf serum

Secondary Incubation

None

Tertiary Incubation

None

Detection

530/30 band pass filter

Results Summary

The antibody stains the kappa light chain on B cells efficiently. In normal B cells, only a portion of cells stain since the others will be lambda-light chain producers. No problem in staining or detecting the signal with this antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to use. GMP antibody. Monoclonal.

The Bad

Need at least 20uL of the antibody.

The Bottom Line

This is a good antibody which was easy to use, but it is needed at 20uL per test. Other antibodies may work as well using less volume per test. The antibody is good and reliable.

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