Tonbo
Ghost Dye™ Violet 510
13-0870-T100
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A viability dye that's commonly used in the lab. Cells can be fixed and persued intracellular staining after.
Flow Cytometry
N/A
1:1000 dilution in FACS buffer for 30 min at RT
Great resolution from negative vs. positive populations
Consistent results across different batches.
Make sure the compensation is set up properly. I used 70% ethanol to kill cells and then stained with this dye.