BD
APC Rat Anti-Mouse IL-4
562045
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I used this antibody for ICS staining for my in vitro Th2 cell differentiation. It works well when cells were fully differentiated and activated by activation cocktail with BrefA.
Flow Cytometry
In vitro mouse Th2 differentiation
1:100 in FACS Buffer at RT for 30 min
N/A
The figure on the left was FMO staining in the absence of IL-4 antibody and the figure on the right was staining with IL-4, suggesting the positive population for IL-4.
Consistent results across different batches
Only works for Flow
It only works well when cells were differentiated well and activated well before staining.