Great Bulk Supply for Making Percoll Gradients

University of Toronto
Chemical/Biomedical Engineering
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cytiva

Product Name:

Percoll Plus

Catalog Number:

17544501

Info: Supplier Page 

We are broadly studying the mechanisms responsible for the establishment and maintenance of immune tolerance.

Experimental Design and Results Summary

Application

Creating percoll density gradients for mTEC isolation

Starting Material

Thymus

Tips

Be very patient when layering different densities of percoll into your gradient, adding layers down the side of the tube is the best way to avoid mining the layers

Results Summary

When creating a three layer percoll gradient for mTEC isolation from the thymus using this product, we are consistently able to recover 3-4 million epithelial cells per mouse with good clean separation of the thymocytes

DOI or PMID #

N/A

Related Categories

Image Gallery

Summary

The Good

Good quality, available in bulk quantities

The Bad

None

The Bottom Line

Highly recommended for creating density gradients for cell isolation

Join the discussion