Anti-MRP1 Antibody with Noisy Background

Genetics
Washington University
post-doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Recombinant Anti-MRP1 antibody

Catalog Number:

ab260038

Clone Number:

N/A

We want to detect MRP level in human cell lines, including WT control and samples after treatment, through western blot. Using this antibody, we are able to find MRP (~180kDa) in the samples, but the background is very noisy.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cell lines

Primary Incubation

1:1000

Blocking Agent

5% Milk in TBST

Secondary Incubation

anti-Rabbit HRP

Tertiary Incubation

none

Detection

ECL

Results Summary

This antibody is able to detect the ~180 Kda protein MRP1 in human cell lines. However, the background the very noisy.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works to find the protein

The Bad

Very strong background

The Bottom Line

It detects the protein, just OK.

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