Fig 2: Effect of AITC on MRP1 expression.(A) Western blot results of Nrf2, DJ-1, MRP1, and HO-1 expression in different groups. GAPDH was used as the internal reference. (B) Quantitative analysis of the results of protein expression in the WT groups. #p < 0.05 vs. control group, *p < 0.05 vs. model group (n = 5). (C) Quantitative analysis of the results of protein expression in the Nrf2-/- groups. #p < 0.05 vs. control group, *p < 0.05 vs. model group (n = 5). Nrf2, nuclear factor erythroid 2-related factor 2; DJ-1, PARK7; MRP1, multidrug resistance-associated protein 1; HO-1, heme oxygenase-1; WT, wild type; AITC, allyl isothiocyanate; NAC, N-acetylcysteine.

Fig 3: Protein expression in the different groups.(A) Western blot results of Nrf2, DJ-1, MRP1, and HO-1 expression in the WT and Nrf2-/- control groups. GAPDH was used as the internal reference. (B) Quantitative analysis of the results of protein expression for each group. Nrf2, nuclear factor erythroid 2-related factor 2; DJ-1, PARK7; MRP1, multidrug resistance-associated protein 1; HO-1, heme oxygenase-1; WT, wild type. *p < 0.05 vs. WT control group (n = 5).

Fig 4: Circ_0058357 affects ABCC1 expression through miR-361-3p. H1299/DDP (a) and A549/DDP (b) cells were transfected with si-circ_0058357 + anti-miR-361-3p, si-circ_0058357 + anti-miR-NC, si-circ_0058357 or si-NC and checked for ABCC1 protein level by western blot. **p < 0.01, ***p < 0.001, ****p < 0.0001

Fig 5: Expression of proteins contributing to chemoresistance and EMT of cancer cells in A549 monolayer culture (2D), SF and SB spheroids. (A) RT-PCR analysis of mRNA levels of growth factors and cytokines. (B) Analysis of protein expression by Western blot. Semiquantitative analysis of the expression levels of vimentin (C), fibronectin (D), α-SMA (E), MRP1 (F), and GSTP1 (G) in 2D A549 cell culture, SF and SB A549 spheroids. Data are shown as means ± SD (n = 3). * p < 0.05, ** p < 0.01 (Mann–Whitney U test).