Dependable Anti-His Probe

Surgery
University of Pittsburgh
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz

Product Name:

His probe

Catalog Number:

sc-8036

Clone Number:

H3

To test the expression of the ADAR1 p150 WT-His plasmid generated by QuikChange II XL SDM Kit, HEK293 cells were transfected with this plasmid. And the expression was detected by Western blot using anti-His probe (sc-8036).

Experimental Design and Results Summary

Applications

Western Blot

Sample

Whole cell lysates

Primary Incubation

1/1000, cold room overnight

Blocking Agent

5% milk

Secondary Incubation

1/10000, room temperature 1 hour

Tertiary Incubation

1/10000, room temperature 1 hour

Detection

ECL

Results Summary

The ADAR1 p150 WT-his plasmid expressed in HEK293

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody works very well.

The Bad

N/A

The Bottom Line

The antibody works for our workflows.

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