His-Tag (H-3) Antibody sc-8036 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemHis-Tag (H-3) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-8036
Quantity200 µg/ml
ApplicationsWB, IP
Conjugate/TagUnconjugated
FormatPurified
TargetHis-Tag
Antibody TypePrimary Antibodies
CloneH-3
ClonalityMonoclonal
Hostmouse
IsotypeIgG1

Questions and Answers

Can this His-Tag antibody be used for detecting recombinant proteins in Western blotting?

Yes, the His-Tag (H-3) Antibody has been shown to be effective for detecting His-tagged proteins in Western blotting applications, as evidenced by its use in various studies, including those that confirm the presence of 6xHis labeled proteins through immunoblotting techniques.

Is this antibody suitable for use in immunoprecipitation experiments?

The His-Tag (H-3) Antibody is suitable for immunoprecipitation, as it has been utilized in studies to pull down His-tagged proteins from cell lysates, facilitating the analysis of protein interactions and functions.

What is the recommended dilution for using this antibody in immunofluorescence?

The His-Tag (H-3) Antibody has been cited in literature at a dilution of 1:300 for immunofluorescence applications, allowing for effective visualization of His-tagged proteins in cellular contexts.

What host species does this antibody originate from, and how does that affect its use?

The His-Tag (H-3) Antibody is derived from mouse, which is important for compatibility with various secondary antibodies. This mouse monoclonal antibody can be effectively used in experiments involving mouse IgG secondary antibodies for detection.

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) Proliferation And Apoptosis Adaptor Protein 15 (PEA15), a potential oncogenic regulator of VHL and HIF1A identified through proteomic analysis in hepatocellular carcinomaCancer CommunicationsApril 8, 2026Yun Seong Jeong, Yun Seong Jeong, Ji-Hyun Shin, Soo Mi Sohn, Sun Young Sohn, Sun Young Yim, Ji-Hoon Shim, Sung Hwan Shim, Sung Hwan Chun, Sunyoung S. Chun, et al.Santa Cruz Biotechnology), anti-hemagglutinin tag (HA) (1:5,000, #901513, BioLegend), anti-histidine (His) probe (1:1,000, sc-8036, Santa Cruz Biotechnology), anti-elongin B (ELOB) (1:1,000, #629302, BioLegend), anti-elongin C (ELOC)

(2) X-linked cancer-associated polypeptide (XCP) from lncRNA1456 modulates PHF8 histone demethylase activity to regulate the epigenome, gene expression, and cellular pathways in breast cancerOncogeneApril 1, 2026Shrikanth S. Gadad, Cristel V. Camacho, Xuan Gong, Micah Thornton, Venkat S. Malladi, Anusha Nagari, Aishwarya Sundaresan, Tulip Nandu, Sneh Koul, Yan Peng, et al.(Invitrogen, PA1-984B RRID:AB_347227), β-tubulin (Abcam, ab6046); RRID:AB_2210370, His Tag (H-3) (Santa Cruz, sc-8036; RRID:AB_627727), Histone H3K9me2 (Cell Signaling, 9753; RRID:AB_659848), Histone H3 (Abcam, ab1791;

(3) INSIG1/2 succination mediated by the moonlighting function of ADSL promotes lipogenesis and liver tumorigenesisNature CommunicationsMarch 15, 2026Yuran Duan, Shuo Wang, Jianyu Liu, Wenxing Qin, Yuli Shen, Yueru Hou, Xue Sun, Yanni Lin, Zhiqiang Hu, Bofei Dong, et al.normal rabbit IgG (sc-2027), mouse ADSL (sc-365623), PKCθ (sc-81534), ERK1/2 (sc-514302), SREBP-1 (sc-13551), His (sc-8036), GST (sc-138), and tubulin (sc-8035) antibodies were obtained from Santa Cruz Biotechnology. ATP-γ-S

(4) OsRALF26 Serves as an Endogenous Signal Recognised by XA21 to Promote Robust and Distal Resistance in RicePlant Biotechnology JournalMarch 11, 2026Oh‐Kyu Kwon, A‐Ram Jeong, Chang‐Jin Parkmembranes (Bio‐Rad). For detection, primary antibodies [anti‐GFP (SC‐9996), anti‐Myc (SC‐47694), anti‐His (SC‐8036), and anti‐GST (SC‐53909); Santa Cruz Biotechnology] and an HRP‐conjugated secondary antibody [mouse

(5) Site-specific phosphorylation of LRP regulates FLC chromatin looping and floweringSci AdvJanuary 1, 2026Sheng Fan, Yu Zhang, Shaobo Zhu, Zhenfeng Teng, Qiqi Zhang, Yuanyuan Yan, Lisha Shenresolved by SDS-PAGE and detected using the anti-HIS antibody (Santa Cruz Biotechnology, catalog no. sc-8036, RRID:AB_627727). Coimmunoprecipitation Seedlings of prp4ka-11 gPRP4KA-3FLAG #5 and F1 crosses of

(6) High-Yield Outer Membrane Vesicles Derived From Probiotics as a Nanoplatform for Precise Treatment and Prophylaxis of Pseudomonas aeruginosa InfectionJ Extracell VesiclesDecember 8, 2025Yadong Yang, Lei Xu, Yongdong Li, Yu Sun, Yanan Tang, Zhibo Xiao, Mengyuan Li, Yi Chen, Yao Wang, Xihui Shenperformed overnight at 4°C using mouse anti‐histidine tag monoclonal antibody as the primary antibody (cat# sc‐8036, Santa Cruz, USA), 1:1000. The membranes were then washed five times with TBST buffer (10‐mM Tris‐HCl,

(7) FAM189A2 activates Hippo signaling pathway by abrogating WWP2-mediated LATS1 ubiquitination, to inhibit the glycolysis and proliferation processes of lung adenocarcinomaJournal of Translational MedicineDecember 5, 2025Bingyu Chen, Xuezhu Rong, Yuheng Feng, Yuekang Hou, Lin Fu, Qiang Han, Yan Song, Xuyong LinProteintech",IB/1:1000IP/1:50 DYKDDDDK-tag,"8146, Cell Signaling Technology66008-2-Ig, Proteintech",IB/1:1000IP/1:50 His-tag,"sc-8036, Santa Cruz","IB/1:200, IP/1:20" Tublin,"11224-1-AP, Proteintech",IB/1:1000 LaminB,"12987-1-AP, Proteintech",IB/1:1000 Primary

(8) Extracellular Domain Shedding of TROP2 Activates EGFR Signaling to Drive Prostate Cancer MetastasisCancer researchDecember 1, 2025Liu S, Hsu EC, Aslan M, Garcia-Marques F, Shen M, Hartono AB, Solano F, Le K, Hwang H, Lee CS, et al.recombinant TECD were incubated with primary antibodies (mouse anti-His-tag, Santa Cruz Biotechnology, sc-8036 and rabbit anti-EGFR, Cell Signaling, #4267) at 1:100 dilution overnight at 4 °C after blocking with

(9) TRIM8-dependent K63-ubiquitinated PGK1 promotes glycolysis and angiogenesis in gastric cancer via interaction with ACAT1Cell Death & DiseaseNovember 3, 2025Anqi Feng, Jianbin Zhang, Zeyu Wang, Zhukai Chen, Kang Fang, Zhaoxing Li, Hanyu Jiang, Zhuyun Leng, Shihan Zhang, Yuan Chu, et al.(Cell Signaling Technology, #2118), anti-Tubulin (Cell Signaling Technology, #5568), anti-His-Tag (Santa Cruz, sc-8036), anti-HA-Tag (Santa Cruz, sc-7392), anti-DYKDDDDK (Flag) Tag (Cell Signaling Technology, #14793). n=

(10) The ubiquitin-binding protein ANKRD13A mediates VCP-dependent mitochondrial outer membrane rupture during PINK1/Parkin-mediated mitophagyJournal of Biological ChemistryNovember 1, 2025Wei-Hua Chu, Yu-Shan Lin, Jing Guo, Wann-Neng Jane, Wong-Jing Wang, Yu-Yang Lin, Pei-Han Liu, Po-Yu Huang, Wei-Chung Chiang(Abcam ab16056, 1:10,000), mouse anti-Flag M2 (Sigma-Aldrich F1804, 1:2000), mouse anti-His (Santa Cruz sc-8036, 1:1000), mouse anti-HSP60 (Proteintech 66041-1-Ig, 1:10,000), rabbit anti-IMMT (Proteintech, 10179-1-AP,

(11) Transient APC/C inactivation by mTOR boosts glycolysis during cell cycle entryNatureOctober 2, 2025Debasish Paul, Derek L. Bolhuis, Hualong Yan, Sudipto Das, Xia Xu, Christina C. Abbate, Lisa M. M. Jenkins, Michael J. Emanuele, Thorkell Andresson, Jing Huang, et al.ab8191, 1:1,000), p21 (BD Biosciences, 556430, 1:1,000), p27 (CST, 3686, 1:1,000), His tag (Santa Cruz, sc-8036, 1:800), anti-DDK/Flag (Sigma-Aldrich, F3165, 1:1,000), p-S6 kinase (CST, 9205, 1:1,000), APC2 (CST,

(12) Structural basis for the recognition and ubiquitylation of type-2 N-degron substrate by PRT1 plant N-recogninNat CommunAugust 21, 2025Woo Seok Yang, Seu Ha Kim, Minsang Kim, Hejeong Shin, Juyeon Lee, Alexander Sandmann, Ohkmae K. Park, Nico Dissmeyer, Hyun Kyu SongSanta Cruz Biotechnology, 1:2000 ratio), and PRT1 was detected using an HRP-conjugated anti-His antibody (sc-8036; Santa Cruz Biotechnology, 1:1000 ratio). Chemiluminescent signals were captured by brief incubation

(13) Integration of biomimetic organoid-on-chip and 2D models advances the mechanistic Understanding of STEAP3-mediated regulation in intestinal viral infectionScientific reportsAugust 20, 2025Chen YW, Chiang HJ, Liu KT, Kao CW, Xie SR, Su CM, Shih YY.;F5879) was purchased from Sigma. Antibodies against STEAP3 (sc-376327), SCARB2 (sc-55571), and His (sc-8036) were from Santa Cruz Biotechnology. Anti-EV-A71(MAB979), anti-Actin (MAB1501), anti-Flag (F3165) antibodies

(14) TBK1 is a signaling hub in coordinating stress-adaptive mechanisms in head and neck cancer progressionAutophagyAugust 1, 2025Kim HJ, Kim HJ, Kim SY, Roh J, Yun JH, Kim CH.(ab56574; 2F3), and anti-PABP (ab21060) were from Abcam. Anti-HA Tag (sc-7392; F7) and anti-His Tag (sc-8036; H-3) antibodies were from Santa Cruz Biotechnology. Anti-ubiquitinated proteins (04–263; FK2) antibody

(15) The RING-domain E3 ubiquitin ligase OsRGLG6 regulates rice grain number and yield via ubiquitination-mediated degradation of OsOTUB1aBIOTECHJuly 23, 2025Jia Chen, Huixia Song, Chenyang Xu, Pengfei Wang, Shuansuo Wangglutathione, pH 8.0). The supernatant was immunoblotted using anti-His (Diluted 1:2000, Santa Cruz, sc-8036) and anti-GST antibodies (Diluted 1:2000, Santa Cruz, sc-138). Co-immunoprecipitation and western blotting The

(16) VCY mediates inhibition of PFV replication via interaction with the transcription activator TasJournal of VirologyJuly 22, 2025Lin Jiang, Chunhua Han, Junshi Zhang, Guoqiang Li, Wentao Qiao, Juan TanSanta Cruz), monoclonal mouse anti-GST (cat. no. sc-138, Santa Cruz), monoclonal mouse anti-His (cat. no. sc-8036, Santa Cruz), monoclonal mouse anti-EGFP (cat. no. AE135, ABclonal Biotechnology, Wuhan, China), horseradish

(17) Dissecting the tRNA Fragment tRF3E-Nucleolin Interaction: Implications in Breast CancerBiomoleculesJuly 21, 2025Maurizio Falconi, Junbiao Wang, Andrea Costamagna, Mara Giangrossi, Sunday Segun Alimi, Emilia Turco, Massimo Bramucci, Luana Quassinti, Rossana Petrilli, Michela Buccioni, et al.electrotransfer. The immunodetection of NCL was achieved with an anti-His tag antibody (His-probe (H-3): sc-8036) from Santa Cruz Biotechnology (Dallas, TX, USA) that was visualized with a peroxidase-conjugated second

(18) Conformational plasticity of a BiP–GRP94 chaperone complexNature Structural & Molecular BiologyJuly 14, 2025Joel Cyrille Brenner, Linda Charlotte Zirden, Lana Buzuk, Yasser Almeida-Hernandez, Lea Radzuweit, Joao Diamantino, Farnusch Kaschani, Markus Kaiser, Elsa Sanchez-Garcia, Simon Poepsel, et al.(Sigma-Aldrich, 01810), doxycycline hyclate (Sigma-Aldrich, DD9891), anti-His5 (Santa Cruz Biotechnologies, SC-8036), anti-Strep (Qiagen, 34850), anti-BiP C50B12 (Cell Signaling, 3177), anti-GRP94 (Proteintech, 14700-1-AP),

(19) Innately Fluorescent Tetravalent Cytotoxic Conjugate TetraFHER2-vcMMAE Engages Aggregation-Dependent Endocytosis of HER2 for Enhanced Intracellular Drug DeliveryJournal of Medicinal ChemistryJuly 10, 2025Natalia Porębska, Aleksandra Chorążewska, Krzysztof Ciura, Adam Pomorski, Artur Krężel, Łukasz OpalińskiReagents The primary antibodies directed against HER2 (ErbB2/HER2, #sc-33684), His-Tag (His-Probe, #sc-8036) and dynamin-2 (#sc-17807) were from Santa Cruz Biotechnology (Dallas, TX). Antitubulin primary antibody

(20) Regulation of NRF2 by stably associated phosphoinositides and small heat shock proteins in response to stressThe Journal of biological chemistryJuly 1, 2025Chen C, Carrillo ND, Chen M, Wen T, Awasthi P, Anderson RA, Cryns VL.(ADI-SPA-801) and αB-crystallin (clone 1B6.1-3G4) from Enzo Lifesciences; HSP27 (sc-13132) and His-tag(sc-8036) from Santa Cruz Biotechnology; PtdIns(4,5)P2 (IgM) from Echelon Biosciences; and HO-1 (A303-662A) from

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9/23/2022Great Anti-His Antibody Liyong Z.HEK293T cells stably expressing control (Ctrl) or USP20 shRNAs were transfected with indicated constructs. After 48 h, cells were treated with MG132 for 6 h before collecting and Ni-NTA beads were ... Read Review
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8/20/2021Dependable Anti-His Probe Liyong Z.To test the expression of the ADAR1 p150 WT-His plasmid generated by QuikChange II XL SDM Kit, HEK293 cells were transfected with this plasmid.

His-Tag (H-3) Antibody

His-Tag (H-3) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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His-Tag (H-3) Antibody from Santa Cruz Biotechnology, Inc.

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