Good Results from Ki67 Antibody

July 28, 2021

BlueskyReddit
Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

Ki-67 Antibody, anti-human/mouse/rat, FITC, REAfinity™

Catalog Number:

130-119-355

Clone Number:

N/A

We wanted to investigate the formation of new hair follicle in murine skin. Ki67 was used to stain the proliferation cells in the follicle

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Murine skin

Primary Incubation

1:20, RT, 30 min

Blocking Agent

5 % Serum; RT, 15 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Signals were detected by an fluorescence microscope

Results Summary

Intranuclear staining was not easy but at the end this antibody worked

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Cheap antibody.

The Bad

N/A

The Bottom Line

We will continue to use this antibody

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