Biolegend
CD44 PE-Cy7
103030
IM7
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I used this anti-mouse/human CD44 PE-Cy7 antibody to stain for antigen-experienced CD8+ T cells in mice. Staining was very good and we observed a clear positive population.
Flow Cytometry
Murine
1:100, 30 minutes at room temperature
Samples were Fc blocked prior to staining. Staining was performed in FACS buffer
N/A
Flow cytometry
Single-cell suspensions were stained using a 1:100 dilution at room temperature for 30 minutes. We successfully identified CD44+ cells from bulk CD8+ T cells in all samples
Good separation between positive and negative populations
None
Reliable antibody for CD44 staining amongst activated CD8+ T cells