Beckman Coulter
CD20-PC7
IM3629U
B9E9
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Browse Similar Products: CD20 Flow Cytometry Antibodies
CD20 is a well-known marker for B cells. Recently, T cells were also found to express CD20 (Schuh E, et al. Features of Human CD3+CD20+ T Cells. J Immunol. 2016 Aug 15;197(4):1111-7). We wanted to detect these cells in human peripheral blood. The selection of the antibody (and the clone) was critical to the success of this project.
Flow Cytometry
Human WBCs
10 minutes, room temp
None
Beckman Coulter 10-C flow cytometer
The staining of B cells was excellent and clean. Positive B cells were easily resolved. For T-cells that express CD20, the resolution was acceptable. As has been shown in the literature, these cells express CD20 at lower levels compared to B cells. The results we got were good enough to identify these cells and quantify them.
The clone identifies CD20+ T cells
It's an IgG2a (higher background)
A very good antibody that identifies CD20+ T cells, and of course B cells. Easy to use and good quality.