Invitrogen
CD45.1 Monoclonal Antibody PE
12-0453-83
A20
Info: Supplier Page
Browse Similar Products: CD45 Flow Cytometry Antibodies
Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. We routinely perform adoptive transfer experiments of donor transgenic T cells (CD45.1 or others) into different CD90.2/CD45.2 WT B6 congenic hosts. In order to analyze donor T cells, we use CD45.1 to distinguish host versus donor. This maker is pan immune marker too.
Flow Cytometry
Murine Splenoyctes
1:500 in FACS Buffer (2% FBS/PBS)
FC Block 1:100ng/ml in FACS
None
BD Celesta
We can readily identify donor T cells and distinguish them from other T cells.
Titrate accordingly
Bright signal
Not too expensive
Highly recommend this antibody if need strong signal to detect donor T cells.