Great Antibody to Analyze CD69 Expression

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BD Biosciences

Product Name:

Anti-Mouse CD69 BUV737

Catalog Number:

612793

Clone Number:

H1.2F3

Acute T cell activation induces strong expression of CD69 as a marker of early activation. We use this antibody in our flow cytometry experiments to assess proper T cell activation.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Tumor samples

Primary Incubation

1:500 in FACS Buffer

Blocking Agent

FC Block in FACS Buffer

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytek Auroa (4 Laser, UV)

Results Summary

We can readily detect robust CD69 surface expression on CD8 T cells found in the tumor.

DOI or PMID #

N/A

Additional Notes

Titrate accordingly

Image Gallery

Summary

The Good

Robust expression is detect to identify positive versus negative

The Bad

None

The Bottom Line

Highly recommend this antibody to detect CD69 expression on T cells.

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