BioLegend BV421 anti-mouse CD3 Ab Review

March 22, 2021

BlueskyReddit
Department of Fundamental Oncology
University of Lausanne
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-mouse CD3 Antibody

Catalog Number:

100228

Clone Number:

17A2

To titrate the antibody, 1 x 10^6 splenocytes from C57BL/6 mouse were stained with: PerCP-Cy5.5 anti-CD4 Ab, BV421 anti-CD3 Ab in the presence of Zombie NIR in 50 μL PBS at 4C for 30 min.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes from C57BL/6 mouse

Primary Incubation

See description

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSRFortessa

Results Summary

To titrate the antibody, 1 x 10^6 splenocytes from C57BL/6 mouse were stained with: PerCP-Cy5.5 anti-CD4 Ab, BV421 anti-CD3 Ab in the presence of Zombie NIR in 50 μL PBS at 4C for 30 min.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Great resolution

The Bad

High Ab concentration (1:50) may be required for optimal staining

The Bottom Line

According to the titration, 1:50 dilution has the best SI.

Share your experience with other scientists. Write a Review! »

Join the discussion