Brilliant Violet 421™ anti-mouse CD3 100228 from BioLegend

Product Specs
ItemBrilliant Violet 421™ anti-mouse CD3
CompanyBioLegend
Price $242.00Supplier Page View Company Product Page
Catalog Number100228
Quantity50 µg
ApplicationsFC - Quality tested, ICC - Verified
Concentrationµg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Conjugate/TagBrilliant Violet 421™
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
ReactivityMouse
IsotypeRat IgG2b, kappa
Clone17A2
HostRat
Antibody TypePrimary
ImmunogenγδTCR-positive T-T hybridoma D1
NCBI Full Gene NameCD3 antigen, gamma polypeptide
NCBI Gene AliasesCtg-3, Ctg3, T3g
ClonalityMonoclonal
TargetCD3

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United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
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Company Profile

Website: biolegend.com

(1) Co-localization of lymph node therapeutics enhances T cell activation and anti-tumor responseBiomaterialsMay 1, 2026Ruochen Shen, Senta M. Kapnick, Camilla Edwards, Ryan A. McIlvaine, Christopher J. Bridgeman, Sean T. Carey, Christopher M. Jewellanalyzed using Flow Jo software. The following antibodies were used: CD11c (BD561241), CD3 (Biolegend 100228), CD8 (Biolegend 100712), CD4 (Biolegend 100512), CD25 (Biolegend 102015), CD44 (Biolegend 103047),

(2) Insufficient erythrocyte-derived S1P: A pathogenic driver and diagnostic biolipid for tumor progressioniScienceApril 17, 2026Zhenjiang Li, Weilun Huang, Zhouzhou Yao, Juncheng Wang, Wenhao Xiao, Qi Guo, Changhan Chen, Tingting Xie, Zhiyu Yang, Qingyang Liu, et al.AB_2687938 HRP-conjugated secondary antibody,Abiowell,Cat# AWS0002; RRID: AB_3676029 Anti-mouse CD3(BV421),BioLegend,Cat# 100228; RRID: AB_2562553 Anti-mouse CD3(PerCP),BioLegend,Cat# 100288; RRID: AB_3683123 Anti-mouse CD45 (APC-Cy7),BioLegend,Cat#

(3) Substituent‐Based Modulation of Self‐Assembly and Immunogenicity of Amphipathic PeptidesAdvanced ScienceMarch 1, 2026Anirban Das, Ushasi Pramanik, Elise M. Brown, Chih‐Yun Liu, Huan Gong, Jonathan Fascetti, Mark Gibson, Samuel Stealey, Silviya P. Zustiak, Cory Berkland, et al.50 µL FWB with OVA‐specific Tetramer (I‐Ab PE, MBL TS‐M710‐1), 50 µL of anti‐CD3 (BV421, Biolegend 100228), and 50 µL of anti‐CD4 (PerCP‐Cy5.5, eBioscience, 45‐0042‐82) for 30 min. Cells were washed 2× with

(4) HDAC1 modulates sepsis-induced immunosuppression by driving the exhaustion of CD8+ T cellsJCI InsightFebruary 23, 2026Liu Di, Jiang-bo Fan, Rui Wang, You Li, Wan-da Bi, Si-yuan Huang, Heng-hai Nie, Xi-feng Feng, Hua-cai Zhang, Juan Du, et al.Pharmingen), APC/Cy7 rat anti-mouse CD45 (30-F11, 561037, BD Pharmingen), and BV421 anti-mouse CD3 (17A2, 100228, BioLegend) antibodies. After washing, the samples were fixed and permeabilized using a Transcription Factor Buffer Set

(5) Bacterial vesicles from intratumoral L. salivarius enhance PD-1 blockade via FPR1-mediated macrophage polarization in gastric cancerCell Reports MedicineFebruary 17, 2026Xiang Yu, Yingxin Ren, Jinlong Luo, Jiaqiang Jiang, Lingzhi Wang, Xinyuan Mao, Yutong Wang, Lang Xie, Yijie Xi, Huilin Huang, et al.RRID:AB_2894427 PE-Cy7 CD45 Clone S18009F,Biolegend,Cat# 157206; RRID:AB_2860726 BV421 CD3 Clone 17A2,Biolegend,Cat# 100228; RRID:AB_2562553 APC-Cy7 CD3 Clone 17A2,Biolegend,Cat# 100222; RRID:AB_2242784 PE CD4 Clone GK1.5,Biolegend,Cat#

(6) A radiolabeled dendrimer non-invasively identifies and tracks innate immune cell activation in a mouse model of experimental autoimmune encephalomyelitisNature CommunicationsJanuary 30, 2026Renesmee C. Kuo, Mackenzie L. Carlson, Samantha T. Reyes, Sydney C. Nagy, Mausam Kalita, Israt S. Alam, Noeen Malik, Isaac M. Jackson, Christopher J. Acosta, Irene N. Falk, et al.AF700 CD45 (BioLegend, San Diego, CA, #147715), PerCP CD19 (BioLegend, #115531), BV421 CD3 (BioLegend, #100228), APC/Fire750 CD11b (BioLegend, #101261), BV570 Ly6G (BioLegend, 127629), PB CD86 (BioLegend, #105021),

(7) Reprogramming tumor microenvironment via systemic delivery of TLR3 agonist and manganese nanoparticleProceedings of the National Academy of SciencesNovember 11, 2025Young Seok Cho, Xingwu Zhou, Xiaoqi Sun, Ziye Wan, Julia Crowther, Mariko Takahashi, Swetha Kodamasimham, Qi Wu, May Thazin Phoo, Youngseo Na, et al.1:100 dilution: anti-CD45-PE-Cy7 (BioLegend, no. 103114; clone 30-F11), anti-CD3-BV421 (BioLegend, no. 100228; clone 17A2), anti-CD4-PE Dazzle (BioLegend, no. 100456; clone GK1.5), anti-CD8-Pacific Blue (BioLegend,

(8) LZTR1 regulates epithelial MHC-I expression via NF-κB1 to modulate CD8+ T cells activationCell DiscovOctober 29, 2025Rundong Jiang, Zhiqin Fang, Yutong Wang, Bo Huang, Junkun Liu, Lam C. Tsoi, Rachael Bogle, Zongbo Zhang, Yehong Kuang, Xin Li, et al.were from Biolegend: anti-mouse CD45 (cat. 103116), anti-mouse CD3 (cat. 100328), anti-mouse CD3 (cat. 100228), anti-mouse TCRβ (cat. 109243), anti-mouse CD4 (cat. 100406), anti-mouse CD8a (cat. 100722), anti-mouse

(9) Synergistic Modulation of the Gut–Brain–Immune Axis by a Dual Lactobacillus Combination in a Murine IBS ModelJournal of Microbiology and BiotechnologyOctober 27, 2025Lim Song, Hanbyeol Lee, Joon-Sun Choi, Seoyeon Park, Hyein Jeong, GwangPyo Koanti-CD11b-PE-Cy7 (BioLegend,101215), anti-F4/80-PE (BioLegend, 123110), anti-CD3-BV421 (BioLegend, 100228), anti-CD4-FITC (BioLegend, 100405), and anti-CD8-PE-Cy7 (BioLegend,100722), anti-CD103-APC (BioLegend,

(10) Design of soluble Notch agonists that drive T cell development and boost immunityCellOctober 16, 2025Rubul Mout, Ran Jing, Mayuri Tanaka-Yano, Emily D. Egan, Helen Eisenach, Martin A. Kononov, Roland Windisch, Mohamad Ali Toufic Najia, Allison Tompkins, Luca Hensch, et al.PE/Dazzle 594,BioLegend,Cat#108747; RRID:AB_2564218 Anti-Mouse CD3 Brilliant Violet 421,BioLegend,Cat#100228; RRID:AB_2562553 Anti-Mouse TCR γ/δ PE,BioLegend,"Cat#107508, RRID:AB_345266" Anti-Mouse CD8a PerCP,BioLegend,Cat#100731;

(11) Whole-genome sequencing reveals rare and structural variants contributing to psoriasis and identifies CERCAM as a risk geneCell GenomicsOctober 8, 2025Kyuto Sonehara, Rei Watanabe, Yutaka Matsumura, Yuichi Mitsui, Yosuke Ogawa, Kaori Odomari, Saori Sakaue, Shinichi Namba, Mariko Komuro, Mio Edamoto, et al.APC anti-mouse IL-17A Antibody,Biolegend,"Cat# 506916, RRID:AB_536018" BV421 anti-mouse CD3 Antibody,Biolegend,"Cat# 100228, RRID:AB_2562553" PE/Cyanine7 anti-mouse TCR γ/δ,Biolegend,"Cat# 118124, RRID:AB_11204423" APC/Cyanine7 anti-mouse CD45

(12) OGFOD1 enables AML chemo- and nutrient stress resistance by regulating protein synthesisCell MetabolismOctober 7, 2025Christina Mayerhofer, Dan Li, Trine Kristiansen, Ernst Mayerhofer, Azeem Sharda, Giulia Schiroli, Karin Gustafsson, Lingli He, Michael Mazzola, Sam Keyes, et al.APC anti-mouse CD4,BioLegend,Cat#: 100516 PE-Cy7 anti-mouse CD8,BioLegend,Cat#: 100722 BV421 anti-mouse CD3,BioLegend,Cat#: 100228 APC-Cy7 anti-mouse B220,BioLegend,Cat#: 103224 BUV395 anti-mouse Gr1,BD Biosciences,Cat#: 566218 BV605

(13) Synovial matrix turnover controls immune cell spatial patterning in inflammation resolutionMolecular Systems BiologySeptember 22, 2025Jean-Baptiste Richard, Anna Hoyle, Molly Bower, Shihong Wu, Leia Worthington, Sarah Davidson, Zofia Varyova, Caroline Morrell, Mathilde Pohin, Barbora Schonfeldova, et al.anti-human CD34,Abcam,ab81289 Rabbit anti-human CD90,Abcam,ab181885 Rat anti-mouse CD3,Biolegend,100228 Rat anti-mouse CD68,Biolegend,137012 Rabbit anti-mouse CD177,R&D Systems,MAB8186 Rat anti-mouse CD31,Biolegend,102520 "Chemicals,

(14) Photoconverted cells allow rapid assessment of vaccine adjuvant potency in miceiScienceJuly 18, 2025Yiwei Zhong, Mingyue Chen, Hongzhe Lin, Cheng Zu, Yue He, Bin WangClone 30-F11,Invitrogen,Cat# 56-0451-82; RRID:AB_891454 Rat anti-CD3(BV421); Clone 17A2,Biolegend,Cat#100228; RRID:AB_2562553 Rat anti-CD8α (PerCP/cy5.5); Clone 53.6.7,Biolegend,Cat#100734; RRID:AB_2075238 Rat

(15) Single atom engineering for radiotherapy-activated immune agonist prodrugsNat CommunJuly 1, 2025Zexuan Ding, Xiaozhe Yin, Yuedan Zheng, Yiyan Li, Huanhuan Ge, Jianshu Feng, Ziyang Wang, Simiao Qiao, Qi Sun, Fashuo Yu, et al.clone YTS156.7.7), anti-mouse CD3-BV421( ≤ 0.25 µg per million cells in 100 µL volume, Biolegend, #100228, clone 17A2), anti-CD16/32 antibody( ≤ 1.0 µg per million cells in 100 µL volume, Biolegend, #101301,

(16) Maternal gut microbiota influences immune activation at the maternal-fetal interface affecting pregnancy outcomeNature communicationsMay 9, 2025Giugliano S, Gatti A, Rusin M, Schorn T, Pimazzoni S, Calanni-Pileri M, Fraccascia V, Carloni S, Rescigno M.11045485, Thermo Fisher Scientific, clone 104, dilution 1:100), CD3 (Cat#: 152312, Biolegend, clone 500-A2, 100228, 100234, Biolegend, clone 17A2, 552774, 561826, BD Pharminghen, clone 145-2C11, dilution 1:100), NK1.1

(17) Gut microbiota-driven pathogenic Th17 cells mediated autoimmune epididymo-orchitis in a mouse model of colitisiScienceApril 23, 2025Qunxiong Zeng, Jin-Chuan Liu, Chen Yang, Minmin Lyu, Dongliang Li, William S.B. Yeung, Philip C.N. Chiu, Tao Zhang, Yong-Gang Duananti-mouse CD44,Biolegend,Cat# 103030; RRID: AB_830787 Brilliant Violet 421™ anti-mouse CD3,Biolegend,Cat# 100228; RRID: AB_2562553 PerCP-Cyanine5.5 anti-mouse GM-CSF,Biolegend,Cat# 505410; RRID: AB_2562376 Brilliant

(18) Boosting tumor homing of endogenous natural killer cells via therapeutic secretomes of chemically primed natural killer cellsJ Immunother CancerMarch 5, 2025Seohyun Cho, Seung Hee Choi, Eunchong Maeng, Hail Park, Ki Seo Ryu, Kyung-Soon ParkPE/Cyanine7-mCD45 (103114), APC/Cyanine7-mNK1.1 (108724), APC-mNKp46 (137608), Brilliant Violet 421-mCD3 (100228), PerCP-mCD4 (100538), and Alexa Fluor 700-mCD8a (100730). All mice were purchased from JA Bio (Gyeonggi-do,

(19) Blimp-1 orchestrates macrophage polarization and metabolic homeostasis via purine biosynthesis in sepsisCell Death DisFebruary 6, 2025Wenjuan Peng, Qiushi Qin, Rui Li, Yujia Liu, Lan Li, Yue Zhang, Liuluan Zhu(65-0112, Tonbo Bioscience San Diego, USA), CD3- eFluor450 (48-0032-82, eBioscience, USA),CD3-BV421 (100228, BioLegend, USA), CD19-PE-Cy7 (115520, BioLegend, USA), CD8-FITC (MA1-10303, eBioscience, USA), CD4-Percp

(20) Multi-omics uncovers immune-modulatory molecules in plasma contributing to resistance exercise-ameliorated locomotor disability after incomplete spinal cord injuryGenome MedFebruary 5, 2025Ren Zhou, Jibao Chen, Yunhan Tang, Chuijin Wei, Ping Yu, Xinmei Ding, Li'ao Zhu, Jiajia Yao, Zengqiang Ouyang, Jing Qiao, et al.103248), CD27-PerCP-Cy5-5 (BioLegend, 124213), CD38-FITC (BioLegend, 102705), CD3-BV421 (BioLegend, 100228), CD4-BV605 (BioLegend, 100547), CD8-PE-CY7 (BioLegend, 100722), CD25-BV785 (BioLegend, 102051), CD69-BV711

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3/22/2021BioLegend BV421 anti-mouse CD3 Ab Review Kung-Chi KaoTo titrate the antibody, 1 x 10^6 splenocytes from C57BL/6 mouse were stained with: PerCP-Cy5.5 anti-CD4 Ab, BV421 anti-CD3 Ab in the presence of Zombie NIR in 50 µL PBS at 4C for 30 min.

Brilliant Violet 421™ anti-mouse CD3 from BioLegend

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Brilliant Violet 421™ anti-mouse CD3 from BioLegend

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