ABCAM
NHERF1- ANTIBODY
AB3452
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I have been working with NHERF1 protein for the past three-four years. I always recommend using Abcam NHERF1 antibody for western IHC and ICC. The specialty of this antibody is its specificity. it will bind to different species of NHERF1.
Western Blot
Human
1:1000, overnight, 4C
5% Milk
1:10000 2 hours 37C
Not applicable
ECL
I have used this antibody to detect NHERF1 protein localization in Human fetal membranes, Human Lung tissues, mouse fetal membranes for both IHC and WB. Also, I used for ICC staining in Human fetal cells, Amnion epithelial cells, and mouse bone marrow mast cells.
33092043
https://pubmed.ncbi.nlm.nih.gov/33361207/ and https://pubmed.ncbi.nlm.nih.gov/33092043/
Specificity to different species
sometimes double band will showed up, may be it is binding to isomer of NHERF-1 protein
Right antibody for NHERF-1 protein studies