Anti-EBP50/NHERF-1 antibody ab3452 from Abcam

Product Specs
ItemAnti-EBP50/NHERF-1 antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab3452
Antibody TypePrimary
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
HostRabbit
ClonalityPolyclonal
Quantity100 UG
IsotypeIgG
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameSLC9A3R1
ReactivityHuman, Mouse
TargetEBP50/NHERF-1

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Mutations in an unrecognized internal NPT2A PDZ motif disrupt phosphate transport and cause congenital hypophosphatemiaBiochemical JournalMay 17, 2023W. Bruce Sneddon, Peter A. Friedman, Tatyana Mamonovawas obtained from R&D Systems (2604-FG-025). Antibodies Antibodies Rabbit polyclonal anti-NHERF1 (ab3452) and monoclonal anti-NHERF1 (Abcam ab31111) were purchased from Abcam. M2-mouse monoclonal anti-HA-agarose

(2) Increased intestinal permeability and downregulation of absorptive ion transporters Nhe3, Dra, and Sglt1 contribute to diarrhea during Clostridioides difficile infectionGut microbesJanuary 1, 2023Peritore-Galve FC, Kaji I, Smith A, Walker LM, Shupe JA, Washington MK, Algood HMS, Dudeja PK, Goldenring JR, Lacy DB.polyclonal anti-Nhe3 (NBP1–82574),Novus Biologicals,1:100,"AB_11038394,56" Rabbit polyclonal anti-NHERF-1 (ab3452),Abcam,1:100,AB_303814 Rabbit polyclonal anti-Sglt1 (576–610),Kaji Lab,0.5 µg/ml,57 Goat anti-rabbit

(3) RGS14 regulates PTH- and FGF23-sensitive NPT2A-mediated renal phosphate uptake via binding to the NHERF1 scaffolding proteinThe Journal of biological chemistryMay 1, 2022Friedman PA, Sneddon WB, Mamonova T, Montanez-Miranda C, Ramineni S, Harbin NH, Squires KE, Gefter JV, Magyar CE, Emlet DR, et al.Mouse and rabbit polyclonal anti-NHERF1 antibodies were purchased from Abcam (catalog nos.: ab9526 and ab3452, respectively). Rabbit polyclonal anti-RGS14 was purchased from Proteintech (catalog no.: 16258-1-AP).

(4) GPR43 regulation of mitochondrial damage to alleviate inflammatory reaction in sepsisAging (Albany NY)September 30, 2021Weiwei Zhang, Wusan Wang, Maodi Xu, Haitang Xie, Zhichen Puab205236, Abcam), PPARγ (1:1000, ab178860, Abcam), NOX-1 (1:1000, ab121009, Abcam), EBP50 (1:1000, ab3452, Abcam), p47phox (1:1000, ab166930, Abcam), NLRP3 (1:1000, 15101, Cell Signaling Technology, Danvers,

(5) Na⁺/H⁺ Exchanger Regulatory Factor 1 Mediates the Pathogenesis of Airway Inflammation in a Murine Model of House Dust Mite–Induced AsthmaThe Journal of ImmunologyMay 15, 2021Ananth K. Kammala, Devika Bahal, Canchai Yang, Reynold A. Panettieri, Rupali Das, Hariharan Subramaniantissues using methods previously described(21). Tissue sections were the probed with the anti-NHERF-1 (ab3452, Abcam, Cambridge, UK) and anti-rabbit secondary antibodies (Vector Laboratories, California, USA) followed

(6) Sodium Hydrogen Exchanger Regulatory Factor-1 (NHERF1) Regulates Fetal Membrane InflammationInternational Journal of Molecular SciencesOctober 20, 2020Ananth Kumar Kammala, Samantha Sheller-Miller, Enkhtuya Radnaa, Talar Kechichian, Hariharan Subramanian, Ramkumar Menonbefore proceeding to staining. Tissue sections were probed with the antihuman NHERF1 antibody (1:200, ab3452, Abcam, Cambridge, UK) overnight at 4 ◦ C, and IHC antirabbit (1:500, Vector Laboratories, CA, USA)

(7) Prognostic and therapeutic implications of NHERF1 expression and regulation in colorectal cancerJournal of Surgical OncologyMarch 1, 2020Premila D. Leiphrakpam, Audrey J. Lazenby, Sanjib Chowdhury, Lynette M. Smith, Michelle Mathiesen, Michael G. Brattain, Jing Wang, Jennifer D. Black, Chandrakanth Are(catalog#2803), and XIAP rabbit monoclonal antibody (catalog#2045). NHERF1 rabbit polyclonal antibody (catalog#ab3452) was obtained from Abcam (Cambridge, MA). Actin rabbit polyclonal antibody (catalog#A2066) was obtained

(8) Circadian clock component PERIOD2 regulates diurnal expression of Na+/H+ exchanger regulatory factor-1 and its scaffolding functionScientific ReportsDecember 1, 2018Yuya Tsurudome et al.Santa Cruz Biotechnology), DBP (1:4000; PM079, MBL), E4BP4 (1:1000; sc-9549, Santa Cruz Biotechnology), NHERF1 (1:1000; ab3452; Abcam, Cambridge, UK), FATP5 (1:1000; sc-70172; Santa Cruz Biotechnology), pan-Cadherin (ab16505, 1:4000;

(9) Proliferation-independent role of NF2 (merlin) in limiting biliary morphogenesisDevelopment (Cambridge, England)April 30, 2018Benhamouche-Trouillet S, O'Loughlin E, Liu CH, Polacheck W, Fitamant J, McKee M, El-Bardeesy N, Chen CS, McClatchey AI.and radixin but not ezrin), anti-E-cadherin (1:500; BD Biosciences 610182), anti-NHERF1 (1:500; Abcam ab3452), anti-HNF4α (1:100; Santa Cruz sc-8987), anti-Par3 (1:100; Millipore 07330), anti-β-catenin (1:100;

(10) Fibroblast-derived HGF drives acinar lung cancer cell polarization through integrin-dependent RhoA-ROCK1 inhibitionCellular SignallingDecember 1, 2017Anirban Datta, Emma Sandilands, Keith E. Mostov, David M. Bryant(Epitomics, 2900-1), GM130 (BD Biosciences, C65120), β-catenin (Santa Cruz, sc-7199), NHERF1 (Abcam, ab3452), Ezrin (BD Biosciences, 610603), Cleaved Caspase 8 (Cell Signaling Technology, 9496), β1-integrin (BD Biosciences

(11) The BEACH protein LRBA is required for hair bundle maintenance in cochlear hair cells and for hearingEMBO reportsNovember 1, 2017Christian Vogl et al.AB1506), rabbit anti-radixin (SigmaAldrich, R3653, batch 044M4752), rabbit anti-Nherf1/EBP50 (Abcam, ab3452), rabbit anti-Nherf2/SLC9A3R2 (Sigma-Aldrich, HPA001672), as well as Atto-488- (Sigma-Aldrich, 49409)

(12) The BEACH Protein LRBA Promotes the Localization of the Heterotrimeric G-protein G_olf to Olfactory CiliaScientific reportsAugust 16, 2017Kurtenbach S, Gießl A, Strömberg S, Kremers J, Atorf J, Rasche S, Neuhaus EM, Hervé D, Brandstätter JH, Asan E, et al.1:500), anti-PSD95 (NeuroMab K28/43 purified 1:12,500). Rabbit polyclonals: anti-Nherf1/EBP50 (Abcam ab3452 1:1000), anti-Stoml3 (Protein-Tech 13316 1:250), anti-Gβ1 (Santa Cruz sc-379 1:10,000), anti-Gβ2 (sc-380

(13) The CFTR trafficking mutation F508del inhibits the constitutive activity of SLC26A9American Journal of Physiology-Lung Cellular and Molecular PhysiologyJune 1, 2017Carol A. Bertrand, Shalini Mitra, Sanjay K. Mishra, Xiaohui Wang, Yu Zhao, Joseph M. Pilewski, Dean R. Madden, Raymond A. Frizzellinteractions in the HEK293 expression system (47). A polyclonal antibody targeting NHERF-1 (aka EBP50, no. ab3452) was purchased from Abcam (Cambridge, MA). The manufacturer qualified this antibody for immunoblotting,

(14) Role of CD34 family members in lumen formation in the developing kidneyDevelopmental biologyOctober 1, 2016Yang Z, Zimmerman SE, Tsunezumi J, Braitsch C, Trent C, Bryant DM, Cleaver O, González-Manchón C, Marciano DK.Par-6b (SCBT, sc-67392, 1:400), Par-3 (Millipore, 07-330), aPKC (SCBT, C-20, 1:300), NHERF1 (Abcam, ab3452), Phalloidin 647 (Invitrogen, 42008A, 1:200). Secondary antibodies were purchased from Jackson ImmunoResearch,

(15) Convergent Signaling Pathways Regulate Parathyroid Hormone and Fibroblast Growth Factor-23 Action on NPT2A-mediated Phosphate TransportThe Journal of biological chemistrySeptember 2, 2016Sneddon WB, Ruiz GW, Gallo LI, Xiao K, Zhang Q, Rbaibi Y, Weisz OA, Apodaca GL, Friedman PA.

(16) Comprehensive proteome analysis of brush border membrane fraction of ileum of ezrin knockdown miceBiomedical research (Tokyo, Japan)January 1, 2016Yoshida S, Fukutomi T, Kimura T, Sakurai H, Hatano R, Yamamoto H, Mukaisho K, Hattori T, Sugihara H, Asano S.

(17) Histone Deacetylase Inhibition Destabilizes the Multi-Potent State of Uncommitted Adipose-Derived Mesenchymal Stromal CellsJournal of Cellular PhysiologyJanuary 1, 2015Amel Dudakovic et al.Millipore), pS473-AKT (1:4,000; AF887; R&D), pT24-FOXO1 (1:2,000; 2599; Cell Signaling), and NHERF1 (1:1,000; Ab3452, Abcam). High throughput RNA-Sequencing Total RNA was isolated from confluent AMSCs in the absence

(18) An unusually powerful mode of low-frequency sound interference due to defective hair bundles of the auditory outer hair cellsProceedings of the National Academy of Sciences of the United States of AmericaJune 24, 2014Kazusaku Kamiya, Vincent Michel, Fabrice Giraudet, Brigitte Riederer, Isabelle Foucher, Samantha Papal, Isabelle Perfettini, Sébastien Le Gal, Elisabeth Verpy, Weiliang Xia, Ursula Seidler, Maria-Magdalena Georgescu, Paul Avan, Aziz El-Amraoui, Christine Petit

(19) Histone deacetylase inhibition promotes osteoblast maturation by altering the histone H4 epigenome and reduces Akt phosphorylationThe Journal of biological chemistryOctober 4, 2013Amel Dudakovic, Jared M Evans, Ying Li, Sumit Middha, Meghan E McGee-Lawrence, Andre J van Wijnen, Jennifer J Westendorf

(20) Ezrin, a membrane cytoskeletal cross-linker, is essential for the regulation of phosphate and calcium homeostasisKidney internationalJanuary 1, 2013Hatano R, Fujii E, Segawa H, Mukaisho K, Matsubara M, Miyamoto K, Hattori T, Sugihara H, Asano S.

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1/27/2021Really Good Antibody ANANTH KUMAR KAMMALAI have been working with NHERF1 protein for the past three-four years. I always recommend using Abcam NHERF1 antibody for western IHC and ICC. The specialty of this antibody is its specificity. it ... Read Review

Anti-EBP50/NHERF-1 antibody from Abcam

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Anti-EBP50/NHERF-1 antibody from Abcam

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