CCL2 Expressed by Fibroblasts

March 26, 2021

BlueskyReddit
UIC
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

PE Mouse Anti-Human MCP-1

Catalog Number:

557066

Clone Number:

N/A

Info: Supplier Page 

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The goal was to detect the expression of CCL2 in skin fibroblasts. This antibody worked well.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Skin fibroblasts

Primary Incubation

1/5, 4C for 15 min

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

100 % of fibroblast expressing CCL2. The cells needed to be fixed and permeabilized before using the antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use

The Bad

None

The Bottom Line

It works well.

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