CXCL1 Expressed by Fibroblasts

UIC
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Pharmingen

Product Name:

Alexa Fluor® 647 Mouse Anti-Human CXCL1 (GROα)

Catalog Number:

566579

Clone Number:

N/A

The goal was to detect CXCL1 expressed by skin fibroblasts. The antibody worked well.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Skin fibroblasts

Primary Incubation

1/40

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

The antibody detected about 50% of fibroblast expressing CXCL1. The cells needed to be fixed and permeabilized before using the antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use

The Bad

None

The Bottom Line

It works.

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