Measuring Histamine Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

EMD Millipore

Product Name:

Anti-Histamine Antibody

Catalog Number:

MAB5408

Clone Number:

N/A

Info: Supplier Page 

Browse Similar Products: Anti-histamine Western Blot Antibodies

Histamine is involved in local immune responses, as well as regulating physiological function in the gut and acting as a neurotransmitter for the brain, spinal cord, and uterus. Histamine is involved in the inflammatory response and has a central role as a mediator of itching. Here we checked the time course effect of histamine expression by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lysate

Primary Incubation

1:1000 dilution prepared in 1x TBST

Blocking Agent

5% milk with 1x TBST

Secondary Incubation

1:4000 dilution prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We found time dependent increase of histamine expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Highly specific and strong signal

The Bad

None

The Bottom Line

This antibody works great for western blot and immunofluorescence staining. Highly recommended.

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