Biolegend
PerCP/Cyanine5.5 anti-mouse/human CD44 Antibody
103032
IM7
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For this experiment I was trying to identify the Effector memory Tcell population (mCD44+CD62L-) so I could then determine their relative receptor levels
Flow Cytometry
Mouse PBMC
30' 4*C
Mouse Fcx Trustain
N/A
Flow
The CD44+ Population is barely distinguishable from the CD44- population when used at 1:200. Without my FMO control I wouldn't have been able to make the gate at all, it is not a distinct separate population. If you absolutely have to use this flurophore, I suggest including an isotype or FMO. Or better yet, switch to a different flurophore. This CD44 clone looks great in BV570 or BV650.
Biolegend shipping is really fast and they have a lot of options
This flurophore isn't really usable for identifying CD44+ cells
Don't use this one, I'd get CD44 in a different Flurophore.