Cell Signaling Technology (CST)
c-Myc
5605
D84C12
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Good antibody for the detection of c-Myc and very sensitive to c-Myc expressions changes
Western Blot
Hela cells, cell lysate
1:1000 milk 1% in PBS 0,05% tween over night
Milk 5% in PBS 0,05% tween
Rabbit 1:3000 milk 1% in PBS 0,05% tween 1 hour
None.
ECL
This antibody is very good and specific. Hela cells WCL has been loaded on a 4-12% SDS-precast gel (1:30h; 180 V) then proteins have been transferred on nitrocellulose membrane using the semi-dry blotting (1h 12V). Blocking with milk 5% in PBS 0,05% tween (45 minutes) was followed by incubation overnight at 4°C degree withc-Myc antibody (1:1000 in milk 1% in PBS 0,05% tween) Then the membrane was washed with 3x 5 minutes with PBS 0,05% tween, followed by a 1-hour incubation with Rabbit secondary antibody 1:3000 in milk 1% in PBS 0,05% tween. The secondary antibody was washed out with a second wash with PBS 0,05% tween (3x 5 minutes). ECL substrate has been used to detect the signal.
Easy and fast to use
It can give a bit of background sometimes, playing with the milk percentage and PBS tween washes is reccomended
Highly suggest buying it