Cell Signaling
Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb
3033
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In our experimental project we determined downstream signaling pathways that are activated by lysophosphatidic acid (LPA). We investigated the effect of LPA on inflammatory transcription factors in microglia cells.
Western Blot
BV2 microglia cells
1:1000 overnight at +4
5% milk
1:5000 goat anti-rabbit, 2 hours at room temperature
N/A
ChemiDoc Development System
These experiments indicated that LPA induced phosphorylation of p65-NF-κB in BV-2 cells.
Very good signal and reproducible results
The best Ab for pp65 detection.