Detection of PKD3 Expression: Successful

October 30, 2020

BlueskyReddit
Molecular Biology and Biochemistry
Medical University of Graz
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

PKD3/PKCν (D57E6) Rabbit mAb

Catalog Number:

5655

Clone Number:

N/A

The purpose of the experiment was to detect the expression of PKD3 in different samples (whole brain lysate, cortex, primary murine microglia, BV2 cells)

Experimental Design and Results Summary

Applications

Western Blot

Sample

Whole brain, cortex, primary murine microglia and BV2 microglia

Primary Incubation

1:1000 overnight at +4

Blocking Agent

5% milk

Secondary Incubation

1:5000 goat anti-rabbit, 2 hours at room temperature

Tertiary Incubation

N/A

Detection

ChemiDoc System

Results Summary

PKD3 seems to be higher expressed in BV2 cells compared to the other 3 samples. However we could see that it is expressed in all the samples tested.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use, reproducible results

The Bad

N/A

The Bottom Line

A good antibody for the detection of PKD3 expression via western blot.

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