PKD3/PKCnu (D57E6) Rabbit mAb 5655S from Cell Signaling Technology

Product Specs
ItemPKD3/PKCnu (D57E6) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number5655S
Quantity100 µl
ApplicationsW, IP
ReactivityH, M, R, Mk, B
TargetPKD3/PKCnu
HostRabbit
NCBI Gene AliasesEPK2, PKC-NU, PKD3, PRKCN, nPKC-NU
ClonalityMonoclonal
NCBI Full Gene Nameprotein kinase D3
Research AreaCa, cAMP and Lipid Signaling
IsotypeIgG
CloneD57E6
Molecular Weight110

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) PRKD3 Overexpression May Improve Survival and Suppresses Proliferation in Colorectal CancerCancer Rep (Hoboken)April 14, 2026Bin Zhang, Chen Chang, Jingli Chen, Yue Cao, Yu Chang, Lele Liu, Wensheng Li, Guorong Wangthe following primary antibodies: rabbit monoclonal anti‐PRKD3 (1:500; Cell Signaling Technology, #5655S) and mouse monoclonal anti‐β‐actin (1:1000; Proteintech, #66009‐1‐Ig). After washing, the membranes

(2) PKD3 localizes to late endosomes to maintain Rab7-dependent endolysosomal homeostasisiScienceSeptember 19, 2025Elena Gutiérrez-Galindo, Katharina Jursik, Yannick Frey, Florian Meyer, Angelika Hausser8188; RRID:AB_10829368 Rabbit mAb anti-PKD3/PKCν (D57E6); WB 1:1000,Cell Signaling Technologies,Cat# 5655; RRID:AB_10695917 Mouse mAb anti-Rab7 (E9O7E); IF 1:100,Cell Signaling Technologies,Cat# 95746; RRID:AB_2800252 Rabbit

(3) Stress and Obesity Signaling Converge on CREB Phosphorylation to Promote Pancreatic CancerMolecular Cancer ResearchMarch 3, 2025Xiaoying Sun, Yaroslav Teper, James Sinnett-Smith, Mineh Markarian, O. Joe Hines, Gang Li, Guido Eibl, Enrique Rozengurt(Cell Signaling Technology Cat# 8188; RRID:AB_10829368, final dilution 1:1000), PKD3 (Cell Signaling Technology Cat# 5655; RRID:AB_10695917, final dilution 1:1000), PKAα/β (Cell Signaling Technology Cat# 3927, RRID:AB_1658217,

(4) Inhibition of protein kinase D and its substrate phosphatidylinositol-4 kinase III beta blocks common human coronavirus replicationMicrobiology SpectrumDecember 5, 2024Huijuan Han, Huan Liu, Rebbeca Steiner, Zhijun Zhao, Zheng-Gen Jinutilized the following primary antibodies: rabbit anti-PKD3/PKCν (D57E6) Rabbit mAb (Cell Signaling; #5655S) and mouse anti-alpha tubulin antibody (Proteintech). Goat anti-rabbit/mouse IgG (H + L)-HRP conjugate

(5) Protein kinase D2 confers neuroprotection by promoting AKT and CREB activation in ischemic strokeNeurobiology of diseaseOctober 15, 2023Connelly JA, Zhang X, Chen Y, Chao Y, Shi Y, Jacob TC, Wang QJ.Type Culture Collection (ATCC, Rockville, MD). The antibodies for PKD1 (90039), PKD2 (8188), PKD3 (5655), AKT (9272), p-S473-AKT (9271), CREB (9197), p-S133-CREB (9198), Cleaved Caspase-3 (9661), and p-S744/748-PKD

(6) Free-Water Diffusion Magnetic Resonance Imaging Differentiates Suicidal Ideation From Suicide Attempt in Treatment-Resistant DepressionBiological Psychiatry: Cognitive Neuroscience and NeuroimagingApril 1, 2023Katie L. Vandeloo, Patricia Burhunduli, Sylvain Bouix, Kimia Owsia, Kang Ik K. Cho, Zhuo Fang, Amanda Van Geel, Ofer Pasternak, Pierre Blier, Jennifer L. Phillips−5]",R,ILF,.048,1.15,0.46–1.82 AD,4,44,"[−38, 27, 10]",L,ATR,.049,1.08,0.39–1.76 AD,5,36,"[−22, −78, −1]",L,IFOF,.050,0.95,0.28–1.61 ADT,1,5655,"[12, −28, −26]",R,CST,.020,1.37,0.66–2.06 ADT,2,1277,"[3, 1, 25]",–,Body of corpus callosum,.030,1.07,0.39–1.74 ADT,3,586,"[−16,

(7) Multiparameter phenotypic screening for endogenous TFEB and TFE3 translocation identifies novel chemical series modulating lysosome functionAutophagyFebruary 1, 2023Carling PJ, Ryan BJ, McGuinness W, Kataria S, Humble SW, Milde S, Duce JA, Kapadia N, Zuercher WJ, Davis JB, et al.anti-PRKD2 (Cell Signaling Technology, 8188) 1:1000; rabbit anti-PRKD3 (Cell Signaling Technology, 5655) 1:1000 and HRP-conjugated anti-ACTB/β-actin (Abcam, ab49900) 1:50,000. For cellular fractionation experiments

(8) Histone deacetylase 5 is a phosphorylation substrate of protein kinase D in osteoclastsBoneJune 1, 2022Meyers CMG, Burciaga SD, Faulkner B, Kazemi P, Cohn JM, Mansky KC, Jensen ED.#4970), P-SRC (Cell Signaling #6943), SRC (Cell Signaling #2123), Protein Kinase D3 (Cell Signaling #5655), normal rabbit IgG (Cell Signaling #2729) and DYKDDDDK (FLAG) Tag (Cell Signaling #14793). 2.5 Mice

(9) Addressing the role of PKD3 in the T cell compartment with knockout miceCell communication and signaling : CCSApril 19, 2022Koutník J, Neururer V, Gruber T, Peer S, Hermann-Kleiter N, Olson WJ, Labi V, Leitges M, Baier G, Siegmund K.1:2000), PKD2 (F-2; sc-374344, 1:1000), both from Santa Cruz Biotechnology, PKD3/PKCν (D57E6) Rabbit mAb #5655 and Phospho-PKD/PKCμ (Ser744/748) Antibody #2054, both from Cell Signaling, 1:1000, sealed in plastic

(10) T cell-intrinsic protein kinase D3 is dispensable for the cells' activationFrontiers in immunologyJanuary 1, 2022Koutník J, Leitges M, Siegmund K.performed as described previously (7) with antibodies specific for PKD3 (Cell Signaling, rabbit mAb #5655, D57E6, 1:1000) and β-actin (Santa Cruz, sc-1615, C-11, 1:2000). Flow cytometry of surface antigens

(11) The Role of Matrix Metalloproteinases in the Progression and Vulnerabilization of Coronary Atherosclerotic PlaquesJournal Of Cardiovascular EmergenciesMarch 1, 2021Diana Opincariu, Nora Rat, Imre Benedekof matrix metalloproteinase type 1: implications for diabetic nephropathy. Endocrinology. 2004;145:5646-5655. doi: 10.1210/en.2004-0436. Knight BE, Kozlowski N, Havelin J, et al. TIMP-1 Attenuates the Development

(12) Evaluation of protein kinase D auto-phosphorylation as biomarker for NLRP3 inflammasome activationPloS oneJanuary 1, 2021Heiser D, Rubert J, Unterreiner A, Maurer C, Kamke M, Bodendorf U, Farady CJ, Roediger B, Bornancin F.Protein Prgl was from BioSource #MBS1177087. Antibodies Anti-phospho PKD #2051, anti-PKD3 (D57E6) #5655, anti-PKD2 (D1A7) #8188, anti-human caspase-1 (D7F10) #3866, anti-cleaved Gasdermin-D (E7H9G) #36425,

(13) The GEF-H1/PKD3 signaling pathway promotes the maintenance of triple-negative breast cancer stem cellsInternational journal of cancerJune 15, 2020Lieb WS, Lungu C, Tamas R, Berreth H, Rathert P, Storz P, Olayioye MA, Hausser A.RRID:AB_390913" Anti-Phospho-(Ser744/748)-PKD,Cell Signaling Technology,"Cat# 2054, RRID:AB_2172539" Anti-PKD3,Cell Signaling Technology,"Cat# 5655, RRID:AB_10695917" Anti-p70 S6 Kinase,Cell Signaling Technology,"Cat# 2708, RRID:AB_390722" Anti-Phospho-(Thr389)-p70

(14) Protein kinase D3 regulates the expression of the immunosuppressive protein, PD‑L1, through STAT1/STAT3 signalingInternational journal of oncologyApril 1, 2020Cui B, Chen J, Luo M, Wang L, Chen H, Kang Y, Wang J, Zhou X, Feng Y, Zhang P.antibodies used for western blot were as follows: PD-L1 (cat. no. 13684; 1:1,000) and PKD3 (cat. no. 5655; 1:1,000) from Cell Signaling Technology; and STAT1 (cat. no. ab109320; 1:10,000), STAT3 (cat. no. ab68153;

(15) Protein kinase Ds promote tumor angiogenesis through mast cell recruitment and expression of angiogenic factors in prostate cancer microenvironmentJournal of Experimental & Clinical Cancer ResearchDecember 1, 2019Wanfu Xu, Jiabi Qian, Fangyin Zeng, Songyu Li, Wenjing Guo, Liping Chen, Guihuan Li, Zhishuai Zhang, Qiming Jane Wang, Fan Dengincubated at 4 °C with the corresponding antibodies overnight, PKD2(CST, 8188,1:2000 for WB), PKD3(CST, 5655,1:2000 for WB), α-tubulin(Ray, RM2007,1:5000 for WB), VEGF(Abclonal, A12303,1:2000 for WB), Erk1/2(Abclonal,

(16) Interplay of PKD3 with SREBP1 Promotes Cell Growth via Upregulating Lipogenesis in Prostate Cancer CellsJournal of CancerJanuary 1, 2019Li L, Hua L, Fan H, He Y, Xu W, Zhang L, Yang J, Deng F, Zeng F.(Millipore, Charlottesville, VA, USA), then incubated overnight at 4℃ with primary antibodies against PKD3(#5655, Cell Signaling Technology), SREBP-1(sc-13551, SantaCruz), SREBP1(sc-366, SantaCruz), polyclonal FASN(A6273,

(17) A Rho signaling network links microtubules to PKD controlled carrier transport to focal adhesionseLifeJuly 20, 2018Eisler SA, Curado F, Link G, Schulz S, Noack M, Steinke M, Olayioye MA, Hausser A."Cell Signaling Technology Cat# 8188S, RRID:AB_10829368", Antibody,PKD3,Cell Signaling,"Cell Signaling Technology Cat# 5655S, RRID:AB_10695917", Antibody,GEF-H1,Cell Signaling,"Cell Signaling Technology Cat# 4076, RRID:AB_2060032", Antibody,phospho-PKD

(18) Lipin-1 determines lung cancer cell survival and chemotherapy sensitivity by regulation of endoplasmic reticulum homeostasis and autophagyCancer medicineJune 1, 2018Fan X, Weng Y, Bai Y, Wang Z, Wang S, Zhu J, Zhang F.

(19) Protein kinase D3 modulates MMP1 and MMP13 expression in human chondrocytesPLOS ONEApril 13, 2018Jonathan Baker, Adrian M. D. Falconer, David J. Wilkinson, G. Nicholas Europe-Finner, Gary J. Litherland, Andrew D. Rowan

(20) Genome-Scale Signatures of Gene Interaction from Compound Screens Predict Clinical Efficacy of Targeted Cancer TherapiesCell SystemsMarch 28, 2018Peng Jiang, Winston Lee, Xujuan Li, Carl Johnson, Jun S. Liu, Myles Brown, Jon Christopher Aster, Xiaole Shirley LiuRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies Rabbit monoclonal anti-PRKD3,Cell Signaling Technology,Cat# 5655S; RRID: AB_10695917 Mouse monoclonal anti-ACTB,Sigma-Aldrich,Cat# A1978; RRID: AB_476692 HRP-linked

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(1)

10/30/2020Detection of PKD3 Expression: Successful Joanna PlastiraThe purpose of the experiment was to detect the expression of PKD3 in different samples (whole brain lysate, cortex, primary murine microglia, BV2 cells)
(1)

8/5/2015Review for Anti-PKD3 Antibody Liyong Z.Cells were washed twice in PBS and harvested with lysis buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM ß-glycerol phosphate, ... Read Review

PKD3/PKCnu (D57E6) Rabbit mAb

PKD3/PKCnu (D57E6) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

Citations (29)

Reviews

PKD3/PKCnu (D57E6) Rabbit mAb from Cell Signaling Technology

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