Immunostaining with Sox2 Antibody

October 06, 2020

Vascular Dysfunction
University of Heidelberg
Postdoctoral Researcher

Overall

Quality of Results

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Company:

R&D

Product Name:

Anti-Sox2

Catalog Number:

AF2018

Clone Number:

N/A

Adult mouse brain tissue was stained with SOX2 antibody to determine the neural stem cell population.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Vibratome sectioned mouse brain tissue

Primary Incubation

1:200, 4 degrees overnight

Blocking Agent

10% Normal donkey serum in PBST (0.5% Triton X 100)

Secondary Incubation

1:500, AF 568

Tertiary Incubation

None

Detection

Immunofluorescence

Results Summary

Sox2 antibody specifically labels neural stem cells in the brain tissue, depending on the region of interest. Sox2 positive cells may be found in the hippocampus or subventricular zone of the adult mouse or in different regions of embryonic cortex and spinal cord during development.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Specific staining, good for thicker sections and brain slices.

The Bad

none

The Bottom Line

Very good antibody, good signal, highly recommended

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