Cell Signaling Technology
Phospho-STAT3
9145
D3A7
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Browse Similar Products: Phospho-STAT3 Immunofluorescence Antibodies
Leptin activates STAT3 though its receptor in the hypothalamus of the brain, which regulates body weight and energy expenditure.
Immunofluorescence
Mouse brain section
1: 250
PBS with 3% normal goat serum with 0.1% tritonX-100
1:000 with Alexa Fluor 488
primary antibody incubated in 4 C for overnight, secondary antibody incubated in room temparature for 1 h.
Confocal microscope
The STAT3 was activated in the ARC, VHM and DMH of mouse hypothalamus 1 h after 60 ug of leptin administration via IP.
Works as expected.
NA
Very strong, specific signal with minimal background.