Abcam
Goat Anti-Rabbit IgG H&L (HRP)
ab205718
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I use this antibody for Western blotting while probing primary antibody is raised in rabbit. Here, I probed Beta actin in THP1 cells using this antibody as the secondary antibody.
Western Blot
THP-1 cells treated with different compounds
1:10000 dilution, 18 hours at 4 degrees with gentle shaking
5% non-fat dry milk in Tris buffer containing 0.1% Tween-20
HRP-conjugated anti-rabbit antibody 1:20000 dilution, 1 hour at room temperature with gentle shaking
Not applicable
ECL based detection
This antibody works great for Western blotting.
High specificity and no background
Nothing
It is a great secondary antibody. No complaints!!