Goat Anti-Rabbit IgG H&L (HRP) ab205718 from Abcam

Product Specs
ItemGoat Anti-Rabbit IgG H&L (HRP)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab205718
Quantity500 µg
ApplicationsELISA, IHC-P, IP, WB
Concentration2 mg/ml
Conjugate/TagHRP
IsotypeIgG
HostGoat
ClonalityPolyclonal
Antibody TypePrimary Antibody
TargetRabbit IgG

Questions and Answers

Can this antibody be used for immunohistochemistry in cancer research?

Yes, the Goat Anti-Rabbit IgG H&L (HRP) has been utilized in immunohistochemistry applications, as demonstrated in studies such as the one by Guo et al. (2021), where it was employed to assess the expression of proteins related to epithelial-mesenchymal transition in ovarian carcinoma tissues.

What is the dilution factor recommended for using this antibody in Western blotting?

The antibody has been used at a dilution of 1:2,000 in Western blot applications, as indicated in multiple studies, including those by Sun et al. (2021) and Xiao et al. (2021), where it was applied to detect various target proteins.

Is this antibody effective for detecting Notch signaling pathway components?

Yes, the Goat Anti-Rabbit IgG H&L (HRP) has been shown to effectively detect proteins involved in the Notch signaling pathway, as evidenced by its use in studies like Jiang et al. (2021), where it was applied to visualize Notch1 expression in osteosarcoma cells.

How has this antibody been cited in the context of colorectal cancer research?

The antibody has been referenced in studies such as that by Li et al. (2020), where it was used in Western blotting to analyze protein expression related to colorectal cancer progression, indicating its relevance in this area of research.

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(1) N6-methyladenosine modification of NEU1 mediated by METTL3 exacerbates angiotensin II-induced atrial fibrillationCell Adhesion & MigrationDecember 31, 2026Zhilan Zhang, Yanbing Luo, Qiuguo Zou, Zelai Moloading control, followed by incubation with corresponding secondary antibodies (goat anti-rabbit, Abcam, ab205718; goat anti-mouse, Abcam ab205719, 1/10000). Immunofluorescence (IF) Atrial tissues underwent fixation

(2) Oridonin-Loaded PDA@Gel@GO Nanocapsules Modulate NLRP3 and Epithelial Repair in ColitisFASEB JApril 30, 2026Wenbo Yuan, Qirui Zhao, Jing Hua, Lingyao Xu, Yufan Wang, Tongtong Xu, Zuchao Du, Liping Wu, Daxiang Cui1 h with an horseradish peroxidase (HRP)‐conjugated goat anti‐rabbit IgG secondary antibody (Abcam, ab205718, 1:3000). Immunoreactive bands were detected using an enhanced chemiluminescence (ECL) kit (Thermo Fisher,

(3) Overexpression of Circ_0004496 is Associated with Pathological Bone Formation in Ankylosing Spondylitis via the miR-145/ACTG1 AxisBalkan Medical JournalApril 30, 2026Yu-Cong Zou, Yong-Sheng Wu, Ting Hu, Hao-Bin ZengRunx2 (ab23981, Abcam, 1:800), GAPDH (ab9485, Abcam, 1:2000), and HRP-conjugated secondary antibody (ab205718, Abcam, 1:1000), all obtained from Abcam (Cambridge, MA, USA). Subcellular fractionation Cytoplasmic

(4) LGALS3BP Links Centrosomes and Mitochondria to Maintain Energetic Fitness and Restrain Compensatory Lipid Catabolic Reprogramming in Hepatocellular CarcinomaCancer Genomics ProteomicsApril 29, 2026Jun Eul Hwang, Hyun Jeong Shim, Hyun Jin Bang, Hyeon-Jong Kim, Sung Yun Jung, Ik-Joo Chung, Sang-Hee Cho, Dae-Hwan KimDetection was carried out using HRP-conjugated secondary antibodies (Goat anti-Rabbit IgG H&L, HRP, Abcam, ab205718; Goat anti-Mouse IgG H&L, HRP, Abcam, ab205719) and enhanced chemiluminescence (ECL) reagents. Fatty

(5) Glimepiride alleviates blood-brain barrier disruption and neuroinflammation in mice with intracerebral haemorrhageAnn MedApril 28, 2026Huizhen Zhou, Qingli Wang, Yang Liu, Xiangyu Zhang, Yun Chen, Pingping Guo, V. Wee Yong, Mengzhou Xuethen washed thrice with TBST for 15 min and incubated with goat anti-rabbit IgG H&L (HRP) (1:5,000, ab205718, Abcam, UK) at room temperature for 1 h. The Amersham Imager 600 (GE Life Sciences, USA) was used to

(6) ADORA2B, deubiquitinated by USP14, facilitates IL-1β-induced chondrocyte apoptosis, ferroptosis and inflammation to accelerate osteoarthritis processMedicine (Baltimore)April 24, 2026Zhi Li, Peng Zhang, Chuanwei Ouyang, Zhou Shen, Genwei Guoanti-GPX4 (1:1000, ab125066, Abcam), anti-GAPDH (1:2500, ab9485, Abcam) and Goat anti-Rabbit IgG (1:2000, ab205718, Abcam) one by one. Protein bands were visualized by ECL reagent (Beyotime), and gray value was evaluated

(7) Nucleoporin TPR integrates MAPK signaling with mitogen-induced transcriptional programsCell Death DisApril 24, 2026Jin Liu, Yifan Zheng, Yi Xiong, Runhua Ma, Zihao Lin, Haikun Lin, Miguel Andújar-Sánchez, Jirina Bartkova, Jian Liu, Marco Foiani, et al.goat anti-mouse IgG H&L (HRP) (Abcam, #ab205719, goat, 1:5000), goat anti-rabbit IgG H&L (HRP) (Abcam, #ab205718, goat, 1:5000). Immunostaining Cells growing on 9 mm coverslips were fixed with 4% formaldehyde (Sinopharm

(8) Spatial multi-omics unveils the monoclonal origin, neuroendocrine plasticity, and microenvironment niches in combined small-cell lung cancerCell Rep MedApril 21, 2026Zhuo Wang, Qing Luo, Jie Wu, Li Lu, Wenjie Ding, Yichun Zhao, Yongfeng Yu, Ruoran Qiu, Ling Zhu, Xinxing Ouyang, et al.(clone SP7),Abcam,Cat# ab16669; RRID:AB_443425 Goat polyclonal anti-rabbit IgG H&L (HRP),Abcam,Cat# ab205718; RRID:AB_2819160 ,, Biological samples,Biological samples,Biological samples ,, Lung cancer tissue

(9) Integrated Bioinformatics Analysis and In Vitro Evidence Support HSP90AA1 as a Candidate Target of Camellia petelotii (Merr.) Sealy in Pulmonary Arterial HypertensionInt J Mol SciApril 21, 2026Xinying Chen, Lipeng Zhou, Chenghao Zhu, Zhirong Sunobtained from Abcam (Cambridge, UK). The HRP-conjugated goat anti-rabbit IgG secondary antibody (Cat. No. ab205718) was purchased from Abcam. RIPA lysis buffer (Cat. No. KGP702-100) and the bicinchoninic acid (BCA)

(10) Respiratory syncytial virus-induced SIGLEC1 upregulation inhibits macrophage autophagy and facilitates inflammatory mediator secretionBMJ Open Respir ResApril 20, 2026Fuhuang Lai, Airong Liao, Kang Xia, Liping Zhang, Hua Yang, Wei Lintemperature with an horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG secondary antibody (Abcam, ab205718). The proteins were visualised using an ECL kit (Cytiva, USA) and imaged using a chemiluminescence imaging

(11) CIDEC impairs mitochondrial fitness and blocks protective autophagy via the inhibition of cGMP/PKG pathway to exert tumor-suppressive effects in breast cancerCell Oncol (Dordr)April 20, 2026Xuchu Jin, Yu Liu, Xinyu Zheng, Yangke Heincubation with horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibody (1:200; Abcam, ab205718) for 1 h at room temperature. The immunoreactivity was visualized using 3,3’-diaminobenzidine (DAB)

(12) Exosomal microRNAs from Alveolar Macrophages Reveal a Protective Role of the Lung Microbiome Against Oncogenic Signaling During PAH ExposureCellsApril 18, 2026Harish Chandra, Brijesh Yadav, Damaris Kuhnell, Scott Langevin, Jacek Biesiada, Mario Medvedovic, Jagjit S. Yadav1:3000 in 5% Milk in TBST for three hours for CD9, and secondary antibody Goat anti-Rabbit IgG H&L (ab205718, Abcam) was added at 1:2000 in 5% BSA in TBST for two hours for TSG101. Following three final washes

(13) Sunitinib induces immunogenic cell death through eIF2α phosphorylation to potentiate immunotherapy in HCCiScienceApril 17, 2026Yimei Gu, Hanbing Mai, Peijian Huang, Hui Yuan, Mingxuan Sun, Huixin Gao, Chenyu Shang, Yangfen Ou, Jizhou Tan, Xianzhang Huang, et al.antibodies were rabbit-derived Mabs, and all secondary antibodies were goat anti-rabbit IgG H&L (HRP) (Cat#ab205718, Abcam). After staining with the primary and secondary antibodies, the corresponding TSA reagents were

(14) Exploratory Evaluation of a Sodium Iodide-Based Root Canal Filling Formulation in a Canine Model of Enterococcus faecalis-Induced Periapical InflammationPharmaceuticsApril 17, 2026Saeromi Jun, Sak Lee, Jong-Soo Kim, Min-Cheol Song, Ji-Sun Shin, Yu-Jin Kim, Jung-Wook Kim, Jung-Hwan Leewere incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (1:500; ab205719 or ab205718, Abcam) for 1 h at room temperature. Immunoreactivity was visualized using 3,3′-diaminobenzidine (DAB)

(15) Protocol for developing a surgically operated model to mimic human glioblastoma resection and recurrenceSTAR ProtocApril 17, 2026Perrine Schneller, Julien Pierson, Joël Daouk, Fabien Rech, Matthieu Doyen, Sophie Pinel, Muriel Barberi-HeyobRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, GFAP,Abcam,#ab207165 IBA1,Abcam,#⁠ab178846 Goat Anti-Rabbit IgG H&L (HRP),Abcam,#⁠ab205718 ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(16) Sanyin decoction alleviates psoriasis by reshaping gut microbiota and modulating the gut–spleen–skin axisFront MicrobiolApril 16, 2026Huangchao Jia, Weiming Zhang, Zhibo Yang, Mengyue Xu, Xuewei Liusections were incubated with an HRP-conjugated goat anti-rabbit IgG secondary antibody (1:500; Abcam, ab205718) for 1 h at room temperature. Immunoreactivity was visualized using a DAB substrate kit (Vector Laboratories,

(17) FGFR2 is a Candidate Immune-Associated Marker of Diabetic Foot Ulcer That Promotes Keratinocyte Function by Activating the PI3K/Akt and MAPK PathwaysMediators InflammApril 15, 2026Hailan Chen, Hongfei Sang, Yi Shi, Jie Pan, Chuang Zhang, Fengrui Leitris buffered saline tween (TBST), the membranes were incubated with the secondary antibody (1:5000, ab205718, Abcam) at room temperature for 2 h. The protein bands were detected using the BeyoECL Plus kit (Beyotime).

(18) Development of Styryl-Modified 3,4-Dihydropyrimidin-2(1H)-ones as Potential Antitumor AgentsChemMedChemApril 14, 2026Konstantinos Panagoulias, Woonghee Kim, Murat Ozdemir, Busra Turan, Adil Mardinoglu, Hasan Turkez, Daniela Trisciuzzi, Orazio Nicolotti, Antonio Di Stefano, Stamatia Vassiliou, et al.(ab8227, Abcam), lamin and α‐tubulin (ab7291, Abcam) were blotted for overnight and Goat Anti‐Rabbit HRP (ab205718) and goat antimouse IgG‐HRP (sc2005, Santa Cruz Biotechnology, Inc.) were blotted for secondary antibody

(19) Many but not all pathogen-associated molecular patterns aggravate neurogenic heterotopic ossification after spinal cord injuryJ Biomed SciApril 10, 2026Selwin G. Samuel, Hsu-Wen Tseng, Bastien Rival, Valérie Barbier, Kavita Bisht, Marjorie Salga, Shrutika M Mate, Whitney Fleming, François Genêt, Sébastien Banzet, et al.(1/1000, ab192256, Abcam) at 4 °C overnight. Membranes were probed with HRP secondary antibody (1/200,000, ab205718, Abcam) at room temperature for 2 h. Enhanced ChemiLuminescence incubation (ECL, 170–5061, Bio-Rad),

(20) Overexpression and knockdown of 5-lipoxygenase regulates the migration and invasion of colorectal cancer cellsOncol LettApril 8, 2026Xuyang Li, Jiletu Huge, Xiaoling Wang, Jing Zhou, Xiudan Li, Xiaoyan Gao, Tiewei Shi, Chunying Baiwith diluted secondary antibody (1:10,000) conjugated with horseradish peroxidase solution (cat. no. ab205718; Abcam) for 1 h at room temperature. After washing, the membrane was visualized with ECL substrate (cat.

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10/22/2020Great Western Blot Antibody Sachin SamuchiwalI use this antibody for Western blotting while probing primary antibody is raised in rabbit.Here, I probed Beta actin in THP1 cells using this antibody as the secondary antibody.

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Goat Anti-Rabbit IgG H&L (HRP) from Abcam

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Goat Anti-Rabbit IgG H&L (HRP) from Abcam

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