Cell Signaling Technologies
Histone H3 (D1H2) XP® Rabbit mAb (HRP Conjugate)
12648S
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Browse Similar Products: Histone H3 Western Blot Antibodies
I used this antibody as a loading control for nuclear fraction of THP1 cell samples treated with different compounds.
Western Blot
THP-1 cells treated with different compounds
1:1000 dilution, 18 hours at 4 degrees with gentle shaking
5% non-fat dry milk in Tris buffer containing 0.1% Tween-20
None
Not applicable
ECL based detection
This antibody is a great nuclear marker. If you are extracting nuclear fraction to study nuclear proteins, I highly recommend using this as a loading control.
High sensitivity
Nothing
This antibody works great for Western blotting purposes.